Large-scale single-cell trapping and imaging using microwell arrays
Large-scale single-cell trapping and imaging using microwell arrays
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DOI：
10.1021/ac0505977
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发表时间：
2005-09-01
影响因子：
7.4
通讯作者：
Folch, A
中科院分区：
文献类型：
--
作者：
Rettig, JR;Folch, A
细胞反应的高通量单细胞测量对于包括药物测试、毒理学和基础细胞生物学在内的各种应用都非常重要。我们提出了一个优化的研究，捕获单细胞与高效率的大阵列的微孔。该方法与标准荧光显微镜设备兼容，不依赖于细胞粘附，但与细胞粘附兼容。我们已经表征了一系列微孔尺寸和接种参数的细胞的微孔占用率，并针对成纤维细胞（作为粘附细胞的模型）和大鼠嗜碱性白血病细胞（作为非粘附细胞的模型）对其进行了优化。我们已经能够获得相差和荧光显微照片与超过18 000个单细胞每图像使用4倍物镜。
High-throughput single-cell measurements of cellular responses are of great importance for a variety of applications including drug testing, toxicology, and basic cell biology. We present an optimization study for trapping single cells with high efficiency in large arrays of microwells. The method is compatible with standard fluorescence microscopy equipment and is not dependent on, but is compatible with, cell adherence. We have characterized microwell occupancy by cells for a range of microwell dimensions and seeding parameters and optimized it for fibroblasts (as a model of adherent cell) and rat basophilic leukemia cells (as a model of nonadherent cell). We have been able to obtain phase-contrast and fluorescence micrographs with more than 18 000 single cells per image using a 4x objective.