The relationship between microbial community structure and functional stability, tested experimentally in an upland pasture soil
The relationship between microbial community structure and functional stability, tested experimentally in an upland pasture soil
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DOI：
10.1007/s00248-002-2043-7
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发表时间：
2004-01-01
影响因子：
3.6
通讯作者：
Fenwick, C
中科院分区：
文献类型：
--
作者：
Griffiths, BS;Kuan, HL;Fenwick, C
从高地牧场收集的土壤在实验中以先前显示的方式改变微生物群落结构。一组土壤被氯仿熏蒸0、0.5、2或24小时，另一组土壤被伽马辐射消毒，然后用10（-2）、10（-4）、10（-6）或10（-8）稀释的未消毒土壤制备的土壤悬浮液接种。孵育8个月后，为了稳定微生物生物量和活性，评估所得微生物群落结构（通过总土壤DNA细菌特异性扩增产物的PCR-DGGE测定）。此外，还确定了功能稳定性（这里定义为植物残留物短期分解对短暂热量或持续铜扰动的抵抗力和恢复力）。还监测了扰动后活性细菌种群的变化（通过RT-PCR-DGGE测定土壤总RNA）。PCR-DGGE图谱显示，操作导致微生物群落结构发生明显变化，但条带数量没有显著减少。微生物群落结构的这些变化与功能稳定性的降低有关。在该高原牧场土壤中观察到的微生物群落结构变化与功能稳定性之间的明确相关性在其他研究中应用相同的方案时并不明显。RT-PCR-DGGE谱只检测到热干扰后活性细菌种群的变化，而不是铜干扰。我们得出结论，分解的功能稳定性与微生物群落的特定成分有关。
Soil collected from an upland pasture was manipulated experimentally in ways shown previously to alter microbial community structure. One set of soil was subjected to chloroform fumigation for 0, 0.5, 2, or 24 h and the other was sterilised by gamma-irradiation and inoculated with a 10(-2), 10(-4), 10(-6), or 10(-8) dilution of a soil suspension prepared from unsterilized soil. Following incubation for 8 months, to allow for the stabilization of microbial biomass and activity, the resulting microbial community structure (determined by PCR-DGGE of bacterial specific amplification products of total soil DNA) was assessed. In addition, the functional stability (defined here as the resistance and resilience of short-term decomposition of plant residues to a transient heat or a persistent copper perturbation) was determined. Changes in the active bacterial population following perturbation (determined by RT-PCR-DGGE of total soil RNA) were also monitored. The manipulations resulted in distinct shifts in microbial community structure as shown by PCR-DGGE profiles, but no significant decreases in the number of bands. These shifts in microbial community structure were associated with a reduction in functional stability. The clear correlation between altered microbial community structure and functional stability observed in this upland pasture soil was not evident when the same protocols were applied to soils in other studies. RT-PCR-DGGE profiles only detected a shift in the active bacterial population following heat, but not copper, perturbation. We conclude that the functional stability of decomposition is related to specific components of the microbial community.