Quantitative proteomics reveals redox-based functional regulation of photosynthesis under fluctuating light in plants
Quantitative proteomics reveals redox-based functional regulation of photosynthesis under fluctuating light in plants
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定量蛋白质组学揭示植物在波动光下光合作用基于氧化还原的功能调控
DOI：
10.1111/jipb.13348
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发表时间：
2022-09-23
影响因子：
11.4
通讯作者：
Jin, Hong-Lei
中科院分区：
文献类型：
--
作者：
Chen, Qi;Xiao, Yixian;Jin, Hong-Lei
光合作用涉及一系列氧化还原反应，是植物细胞中活性氧的主要来源。波动的光（FL）水平，这通常发生在自然环境中，影响光合作用，然而，很少有人知道的具体影响FL对光合作用的氧化还原调节。在这里，我们进行了全球定量映射的拟南芥半胱氨酸巯基氧化还原蛋白质组在恒定的光和FL条件下。我们在4350种蛋白质中鉴定了8857种氧化还原开关硫醇，以及1501种根据光照条件进行差异修饰的蛋白质。值得注意的是，与光合作用相关的蛋白质，特别是光系统I（PSI），是可操作的巯基转换热点。野生型A.结果表明，拟南芥对FL的抗性导致PSI丰度、稳定性和活性降低。有趣的是，响应于PSI光损伤，更多的PSI组装因子PSA 3动态地切换到还原状态。此外，PSA 3中的Cys199和Cys200位点对于其全部功能是必需的。此外，硫氧还蛋白m（Trx m）蛋白在PSA 3的氧化还原开关中起作用，并且是PSI活性和光合作用所需的。因此，本研究揭示了一种机制，氧化还原为基础的调节PSI下FL，并提供了深入了解光合作用在不断变化的环境中的动态驯化。
Photosynthesis involves a series of redox reactions and is the major source of reactive oxygen species in plant cells. Fluctuating light (FL) levels, which occur commonly in natural environments, affect photosynthesis; however, little is known about the specific effects of FL on the redox regulation of photosynthesis. Here, we performed global quantitative mapping of the Arabidopsis thaliana cysteine thiol redox proteome under constant light and FL conditions. We identified 8857 redox-switched thiols in 4350 proteins, and 1501 proteins that are differentially modified depending on light conditions. Notably, proteins related to photosynthesis, especially photosystem I (PSI), are operational thiol-switching hotspots. Exposure of wild-type A. thaliana to FL resulted in decreased PSI abundance, stability, and activity. Interestingly, in response to PSI photodamage, more of the PSI assembly factor PSA3 dynamically switches to the reduced state. Furthermore, the Cys199 and Cys200 sites in PSA3 are necessary for its full function. Moreover, thioredoxin m (Trx m) proteins play roles in redox switching of PSA3, and are required for PSI activity and photosynthesis. This study thus reveals a mechanism for redox-based regulation of PSI under FL, and provides insight into the dynamic acclimation of photosynthesis in a changing environment.