Sulfation of polysaccharides generates potent and selective inhibitors of human immunodeficiency virus infection and replication in vitro.
Sulfation of polysaccharides generates potent and selective inhibitors of human immunodeficiency virus infection and replication in vitro.
复制标题
多糖的硫酸化可在体外产生人类免疫缺陷病毒感染和复制的有效且选择性的抑制剂。
DOI：
10.20772/cancersci1985.78.11_1164
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
N. Yamamoto
中科院分区：
文献类型：
--
作者：
H. Nakashima;O. Yoshida;T. Tochikura;T. Yoshida;T. Mimura;Y. Kido;Y. Motoki;Y. Kaneko;T. Uryu;N. Yamamoto
用携带HTLV-I的MT-4细胞株体外检测了葡聚糖、木呋喃和核呋喃及其硫酸酯多糖对人类免疫缺陷病毒(HIV)感染性和复制的抑制作用。葡聚糖硫酸盐(分子量34×10(3))、木呋喃硫酸盐和核呋喃硫酸盐在浓度大于10微克/毫升时完全阻止HIV诱导的细胞病变效应(CPE)，在大于100微克/毫升浓度时完全阻止葡聚糖硫酸盐(分子量7×10(3))。然而，在所测试的任何浓度下，非硫酸化合物都不能阻止它们。通过检测感染的MT-4细胞中HIV特异性抗原的表达，证实了这些多糖的抗HIV作用。在与MOLT-4和MOLT-4/HIVHTLV-IIIB细胞共培养时，100微克/毫升这些硫酸盐化合物可完全抑制多核细胞的形成。1000微克/毫升的葡聚糖硫酸盐对未感染的MT-4细胞的生长抑制率为20-30%，而硫酸葡聚糖、硫酸木糖和硫酸核苷呋喃对硫酸多糖的抑制作用不明显，能有效地抑制禽成髓细胞增多症病毒和HIV的逆转录酶活性。
The inhibitory effects of several polysaccharides, dextran, xylofuranan, and ribofuranan, and their sulfated counterparts on the infectivity and replication of human immunodeficiency virus (HIV) were examined by using an HTLV-I-carrying cell line, MT-4, in vitro. Dextran sulfate (Mw 34 X 10(3], xylofuranan sulfate, and ribofuranan sulfate completely prevented HIV-induced cytopathic effects (CPE) at concentrations greater than 10 micrograms/ml and dextran sulfate (Mw 7 X 10(3] at concentrations greater than 100 micrograms/ml. However, the non-sulfated compounds did not prevent them at any concentration tested. The anti-HIV effect of these polysaccharides was confirmed by measuring HIV-specific antigen expression in infected MT-4 cells. In cocultures with MOLT-4 and MOLT-4/HIVHTLV-IIIB cells, formation of multinucleated cells was completely inhibited in the presence of 100 micrograms/ml of these sulfated compounds. Dextran sulfate showed 20-30% growth inhibition of uninfected MT-4 cells at 1000 micrograms/ml but dextran sulfate, xylofuranan sulfate, and ribofuranan sulfate showed no effect on sulfated polysaccharides efficiently inhibited the reverse transcriptase activity of avian myeloblastosis virus and HIV.