Intraarterially Delivered Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Canine Cerebral Ischemia
Intraarterially Delivered Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Canine Cerebral Ischemia
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DOI：
10.1002/jnr.22162
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发表时间：
2009-12-01
影响因子：
4.2
通讯作者：
Kim, Hwi-Yool
中科院分区：
文献类型：
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作者：
Chung, Dai-Jung;Choi, Chi-Bong;Kim, Hwi-Yool
本研究观察了人脐血来源的间充质干细胞(HUCB来源的MSCs)在犬血栓栓塞性脑缺血模型中的作用。用血栓栓塞法阻断大脑中动脉，造成脑缺血模型。HUCBC组(n=5)于缺血诱导后1d经基底动脉血管内介入移植1×106个人脐血间充质干细胞。对照组(n=5)注射磷酸盐缓冲液(PBS)，与HUCBC组相同。神经行为检查显示，HUCBC组恢复较早。磁共振图像分析显示，HUCBC组在脑缺血诱导后1周时脑梗塞体积缩小，而对照组在1周时脑梗塞体积增大。移植细胞已分化为神经元和星形胶质细胞，并可在von Willebrand因子(VWF)阳性的内皮细胞及其周围观察到。移植后4周，人脐血来源的MSCs表达脑源性神经营养因子和血管内皮生长因子等神经保护因子。移植的细胞通过减少梗死灶的体积和更早地从神经缺陷中恢复来证明它们的有效性。这些结果表明，脐带血来源的MSCs动脉内移植可用于临床治疗脑缺血。(C)2009年Wiley-Liss，Inc.
The present study examined the effects of human umbilical cord blood-derived mesenchymal stem cells (HUCB-derived MSCs) delivered through the basilar artery in a canine thromboembolic brain ischemia model. Cerebral ischemia was induced through occlusion of the middle cerebral artery by injecting thrombus emboli into 10 beagles. In the HUCBC group (n = 5), 1 X 106 HUCB-derived MSCs were transplanted through the basilar artery 1 day after ischemic induction using an endovascular interventional approach. In the control group (n = 5), phosphate-buffered saline (PBS) was injected in the same manner in as the HUCBC group. Upon neurobehavioral examination, earlier recovery was observed in the HUCBC group. The HUCBC group showed a decrease in the infarction volume at 1 week after cerebral ischemic induction, whereas the control group showed an increase in the infarction volume at 1 week, by magnetic resonance image analysis. Transplanted cells had differentiated into neurons and astrocytes and were observed in and around endothelial cells that were positive for von Willebrand factor (vWF). HUCB-derived MSCs expressed neuroprotective factors, such as brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF), at 4 weeks after the transplantation. The transplanted cells demonstrated their efficacy by reducing the infarction lesion volume and through earlier recovery from the neurological deficit. These results suggest that intraarterial transplantation of HUCB-derived MSCs could be useful in clinical treatment of cerebral ischemia. (C) 2009 Wiley-Liss, Inc.