Composition of Anopheles coluzzii and Anopheles gambiae microbiota from larval to adult stages
Composition of Anopheles coluzzii and Anopheles gambiae microbiota from larval to adult stages
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DOI：
10.1016/j.meegid.2014.09.029
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发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Morlais, Isabelle
中科院分区：
文献类型：
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作者：
Gimonneau, Geoffrey;Tchioffo, Majoline T.;Morlais, Isabelle
在未成熟的生命阶段，疟疾蚊子暴露于水生栖息地的各种微生物和污染物。虽然先前的研究表明，环境暴露塑造了成年蚊子的微生物群落结构，但大多数报告都集中在实验室实验和单一的蚊子上皮细胞，即肠道。在这项研究中，我们调查了自然条件下的繁殖地点对按蚊coluzzii和冈比亚按蚊微生物区系发育的影响。我们使用多重16S rRNA基因焦磷酸测序对水生栖息地的细菌群落进行了表征，在表面微层和次表层水位，以新鲜出现的成年蚊子，我们分别分析了与成年个体，中肠，卵巢和唾液腺的不同上皮细胞相关的微生物群。我们发现，细菌群落在水生栖息地的分布不同，根据水收集的深度。幼虫肠道细菌组成的个体间差异较大，但同一繁殖地点的成蚊肠道菌群非常相似。尽管在新鲜出现的AN的不同上皮之间突出了细菌丰度的一些差异。coluzzii和安氏隐翅虫An.冈比亚，我们研究的一个有趣的特征是整个细菌群落的特殊相似性。我们的研究结果要求进一步研究不同组织中的细菌种群动态，以确定蚊子寿命期间每个微生物群的独特特征，并确定某些关键门或物种与昆虫生活史特征之间的特定相互作用。(C)2014 Elsevier B.V.版权所有
During their immature life stages, malaria mosquitoes are exposed to a wide array of microbes and contaminants from the aquatic habitats. Although prior studies have suggested that environmental exposure shapes the microbial community structure in the adult mosquito, most reports have focused on laboratory-based experiments and on a single mosquito epithelium, the gut. In this study, we investigated the influence of the breeding site on the development of the Anopheles coluzzii and Anopheles gambiae microbiota in natural conditions. We characterized bacterial communities from aquatic habitats, at surface microlayer and subsurface water levels, to freshly emerge adult mosquitoes using multiplexed 16S rRNA gene pyrosequencing and we separately analyzed the microbiota associated with the different epithelia of adult individual, midguts, ovaries and salivary glands. We found that the distribution of bacterial communities in the aquatic habitats differed according to the depth of water collections. Inter-individual variation of bacterial composition was large in larvae guts but adult mosquitoes from a same breeding site shared quite similar microbiota. Although some differences in bacterial abundances were highlighted between the different epithelia of freshly emerged An. coluzzii and An. gambiae, an intriguing feature from our study is the particular similarity of the overall bacterial communities. Our results call for further investigations on the bacterial population dynamics in the different tissues to determine the distinctive characteristics of each microbiota during the mosquito lifespan and to identify specific interactions between certain key phyla or species and the insect life history traits. (C) 2014 Elsevier B.V. All rights