GENETIC MAP FOR COWPEA STRAIN OF TMV
GENETIC MAP FOR COWPEA STRAIN OF TMV
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DOI：
10.1016/0042-6822(76)90411-6
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发表时间：
1976-01-01
期刊：
影响因子：
3.7
通讯作者：
ISRAEL, HW
中科院分区：
文献类型：
--
作者：
BEACHY, RN;ZAITLIN, M;ISRAEL, HW
TMV（烟草花叶病毒）的豇豆菌株在受感染的组织（绿豆、菜豆、烟草）中产生多种大小的杆状体，其中包括含有衣壳蛋白基因的短杆状体。当全长杆在 60°C 下孵育时。 C与十二烷基硫酸钠，每个部分破坏的病毒颗粒仅在一端可见一条RNA链。这种部分剥离的病毒 (PSV) 用 RNase 处理，然后用去污剂和苯酚处理，以回收受未剥离蛋白 (PROT-RNA) 保护的 RNA 部分。 PSV、长杆状RNA、短杆状RNA和来自天然存在的中等长度杆状体的RNA (I2-RNA)，但不是PROT-RNA，用缬氨酸进行酶促氨酰化。这种 TMV 豇豆品系的长杆与普通品系的长杆一样，明显从 RNA 的 3'' 端剥离； PROT-RNA 必须来自 5'' 端。 PSV、长杆状RNA和短杆状RNA，但不是PROT-RNA，在与来自豇豆菌株TMV感染组织的熔化双链RNA的分子杂交反应中与标记的短杆状RNA完成。 I2-RNA 先前被证明含有短杆状 RNA 的核苷酸序列。因此，短杆状RNA和中间杆状RNA可以源自靠近3''末端的那些长杆状RNA核苷酸序列。表观分子量为 1.4 倍的 PROT-RNA。 106 和长杆状 RNA 都指导表观分子量为 130,000 的多肽的合成[在小麦胚芽无细胞系统中]。结果与该高MW多肽的基因接近长杆状RNA的5''末端、衣壳蛋白基因接近3''末端以及I2-RNA易位产物（MW约29,000）位于其间一致。
A cowpea strain of TMV [tobacco mosaic virus] engenders rods of several sizes in infected tissues [Vigna sinensis, Phaseolus vulgaris, Nicotiana tabacum], including a short rod which contains the capsid protein gene. When full length rods were incubated at 60.degree. C with sodium dodecyl sulfate, each partially disrupted virion had an RNA strand visible at 1 end only. This partially stripped virus (PSV) was treated with RNases and then with detergent and phenol to recover the portion of the RNA protected by the unstripped protein (PROT-RNA). PSV, long rod RNA, short rod RNA and RNA (I2-RNA) from a naturally occurring rod of intermediate length, but not PROT-RNA, were enzymatically aminoacylated with valine. The long rods of this cowpea strain of TMV, like those of the common strain, are apparently stripped from the 3''-end of the RNA; the PROT-RNA must be derived from the 5''-end. PSV, long rod RNA and short rod RNA, but not PROT-RNA, completed with labeled short rod RNA in molecular hybridization reactions with melted double-stranded RNA from cowpea strain TMV-infected tissue. I2-RNA previously was shown to contain nucleotide sequences of the short rod RNA. Thus, the short and intermediate rod RNA may be derived from those long rod RNA nucleotide sequences which are close to the 3''-terminus. PROT-RNA of apparent MW 1.4 .times. 106 and long rod RNA both directed the synthesis of polypeptides of apparent MW 130,000 [in a wheat germ cell free system]. The results are consistent with the gene for this high MW polypeptide being close to the 5''-terminus of the long rod RNA, the capsid protein gene being close to the 3''-terminus, and the I2-RNA translocation product (MW .apprx. 29,000) being between.