AN OLIGOSACCHARIDE OF THE O-LINKED TYPE DISTINGUISHES THE FREE FROM THE COMBINED FORM OF HCG ALPHA-SUBUNIT
AN OLIGOSACCHARIDE OF THE O-LINKED TYPE DISTINGUISHES THE FREE FROM THE COMBINED FORM OF HCG ALPHA-SUBUNIT
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DOI：
10.1016/0006-291x(84)91228-2
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
RUDDON, RW
中科院分区：
文献类型：
--
作者：
COLE, LA;PERINI, F;RUDDON, RW
恶性滋养层细胞产生游离的α。除了α亚基之外，与β-亚单位为hCG [人绒毛膜促性腺激素]。自由α通过凝胶色谱法和SDS-PAGE [十二烷基硫酸钠-聚丙烯酰胺凝胶电泳]测得的α大于组合的α。而不能与β相关联，亚基形成hCG。一种胰蛋白酶片段，代表氨基酸残基36-42，来源于游离α-淀粉酶。大于来自组合α的相应片段。在神经氨酸酶处理后，来自游离α的片段被去除。结合的花生凝集素琼脂糖，其对O-连接寡糖中发现的Gal β 1 -3GalNAc具有特异性。该片段还含有Gal和GalNAc（和较少量的GlcNAc），如通过糖苷酶敏感性和氨基糖分析所确定的。去除该胰蛋白酶片段消除了游离和结合的α-淀粉酶之间的大小差异。亚单位。
JAR malignant trophoblast cells produce a free .alpha. subunit in addition to an .alpha. combined with .beta.-subunit as hCG [human chorionic gonadotropin]. The free .alpha. is larger by gel chromatography and SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis] than combined .alpha. and is unable to associate with .beta.-subunit to form hCG. A tryptic fragment, representing amino acid residues 36-42, derived from free .alpha. was larger than the corresponding fragment from combined .alpha.. After neuraminidase treatment, the fragment from free .alpha. bound peanut lectin agarose, which is specific for Gal.beta.1-3GalNAc as found in O-linked oligosaccharides. The fragment also contained Gal and GalNAc (and a lesser amount of GlcNAc) as determined by glycosidase sensitivity and amino sugar analyses. Removal of this tryptic fragment ablated the size difference between free and combined .alpha.-subunits.