vvd is required for light adaptation of conidiation-specific genes of Neurospora crassa, but not circadian conidiation.
vvd is required for light adaptation of conidiation-specific genes of Neurospora crassa, but not circadian conidiation.
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DOI：
10.1006/fgbi.2001.1264
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发表时间：
2001-04
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通讯作者：
Lori Bailey Shrode;Zachary A. Lewis;Lori D. White;D. Bell-Pedersen;D. Ebbole
中科院分区：
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作者：
Lori Bailey Shrode;Zachary A. Lewis;Lori D. White;D. Bell-Pedersen;D. Ebbole
con-10和con-6是粗糙脉孢菌的两个分生孢子（con）基因，其基于它们在大分生孢子柄发育期间的优先表达而被鉴定。它们也受到其他几种独立于发育的环境刺激的调节，包括光的瞬时诱导。我们在突变体筛选中鉴定了vivid（vvd）的一个等位基因，该筛选旨在获得con-10表达改变的菌株。VVD突变体对光的反应显示出增强的类胡萝卜素色素沉着。此外，con-10和con-6显示对光诱导的反应增强。我们测试了vvd突变体中光响应生物钟的功能，没有发现分生孢子的昼夜节律或关键生物钟成分频率（frq）的光调节的重大缺陷。我们的结论是，vvd主要涉及光依赖的基因抑制过程，称为光适应。虽然已知有许多基因产物控制真菌的光诱导，但vvd是第一个被证明在适应恒定光照中起作用的基因。
con-10 and con-6 are two of the conidiation (con) genes of Neurospora crassa that were identified based on their preferential expression during macroconidiophore development. They are also regulated by several other environmental stimuli independent of development, including a transient induction by light. We identified an allele of vivid (vvd) in a mutant screen designed to obtain strains with altered expression of con-10. vvd mutants display enhanced carotenoid pigmentation in response to light. In addition, con-10 and con-6 show a heightened response to photoinduction. We tested the function of the light-responsive circadian clock in the vvd mutant and found no major defect in the circadian rhythm of conidiation or light regulation of a key clock component, frequency (frq). We conclude that vvd is primarily involved in a process of light-dependent gene repression, called light adaptation. Although a number of gene products are known to control light induction in fungi, vvd is the first gene shown to have a role in adaptation to constant light.