Rb1cc1 is critical for myoblast differentiation through Rb1 regulation
Rb1cc1 is critical for myoblast differentiation through Rb1 regulation
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DOI：
10.1007/s00428-004-1183-1
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发表时间：
2005-06
期刊：
影响因子：
3.5
通讯作者：
R. Watanabe;T. Chano;H. Inoue;T. Isono;O. Koiwai;H. Okabe
中科院分区：
文献类型：
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作者：
R. Watanabe;T. Chano;H. Inoue;T. Isono;O. Koiwai;H. Okabe
Rb1诱导卷曲螺旋1（Rb1cc1）高水平表达与人胚胎肌肉骨骼细胞的成熟有关。为了阐明Rb1cc1在肌肉分化中的分子作用，我们研究了Rb1cc1和其他调节小鼠胚胎组织和C2C12成肌细胞分化的基因的表达。我们还评估了RNA干扰（RNAi）介导的Rb1cc1敲低对C2 C12成肌细胞分化的影响。Rb1cc1、Rb1和肌球蛋白重链（Myhc）在小鼠胚胎肌肉中表达。在C2C12成肌细胞分化过程中，Rb 1cc1和Rb 1的同步表达预测了Myhc的表达。RNAi介导的Rb1cc1基因敲低导致Rb1抑制，C2C12成肌细胞不能分化。这些结果表明，Rb1cc1是Rb1通路的有效调节剂，并且是在肌肉分化中起关键作用的新型介质。因此，Rb1cc1表达是肌源性分化的先决条件。
Rb1-inducible coiled-coil 1 (Rb1cc1) expressed at high levels is associated with the maturation of human embryonic musculoskeletal cells. To clarify the molecular role of Rb1cc1 in muscular differentiation, we investigated the expression of Rb1cc1 and other genes that regulate differentiation in murine embryonic tissues and in C2C12 myoblasts. We also evaluated the effects of RNA interference (RNAi)-mediatedRb1cc1knockdown on C2C12 myoblast differentiation. After Rb1cc1, Rb1 and myosin heavy chain (Myhc) were expressed in mouse embryonic muscles. The synchronous expression ofRb1cc1andRb1predictedMyhcexpression during C2C12 myoblast differentiation. RNAi-mediated knockdown ofRb1cc1led toRb1suppression, and C2C12 myoblasts failed to differentiate. These results indicated that Rb1cc1 is a potent regulator of the Rb1 pathway and a novel mediator that plays a crucial role in muscular differentiation. Rb1cc1 expression is, thus, a prerequisite for myogenic differentiation.