Reproductive potential of mosquitofish is reduced by the masculinizing effect of a synthetic progesterone, gestodene: Evidence from morphology, courtship behaviour, ovary histology, sex hormones and gene expressions
Reproductive potential of mosquitofish is reduced by the masculinizing effect of a synthetic progesterone, gestodene: Evidence from morphology, courtship behaviour, ovary histology, sex hormones and gene expressions
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DOI：
10.1016/j.scitotenv.2020.144570
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发表时间：
2021-01-21
影响因子：
9.8
通讯作者：
Leung, Jonathan Y. S.
中科院分区：
文献类型：
--
作者：
Chen, Shanduo;Lin, Canyuan;Leung, Jonathan Y. S.
越来越多地使用合成激素，特别是孕激素，用于医疗应用，由于其潜在的内分泌干扰作用，可能会减少水生生物的生殖产出，引起越来越多的关注。以食蚊鱼（Gambusia affinis）为模型鱼，研究了孕二烯酮（gestodene，GES）对性激素合成相关基因表达的影响，以及对形态特征、求偶行为和卵母细胞发育的影响。在暴露于环境相关浓度（2.96，32.9和354 ng L-1）的GES 40天后，我们发现，GES，特别是在354 ng L-1，诱导雌性鱼的雄性化，表现为体重与长度的比例减少和生殖足（即雄鱼的臀鳍）的发育。因此，男性表现出较少的亲密关系和交配的兴趣对GES暴露的女性，减少了时间花在出席，以下和交配行为。虽然卵母细胞发育似乎不受GES的影响，但精原细胞在卵巢中发育。GES的所有上述雄性化作用与睾酮水平升高和雌二醇水平降低相关，这是由上调雄激素受体基因（Ar α和Ar β）驱动的。总的来说，我们的研究结果表明，孕激素可能会破坏水生生物的生殖潜力，因此它们在孕激素污染的环境中的持久性。(C)2021爱思唯尔有限公司版权所有。
The ever-increasing use of synthetic hormones, especially progestins, for medical applications has drawn growing concerns due to their potential endocrine disrupting effects that may diminish the reproductive outputs of aquatic organisms. Using mosquitofish (Gambusia affinis) as a model species, we tested whether gestodene (GES), a commonly used progestin, can alter the expressions of genes associated with sex hormone synthesis and cause ensuing changes in morphological features, courtship behaviour and oocyte development. After exposing to GES at environmentally relevant concentrations (2.96, 32.9 and 354 ng L-1) for 40 days, we found that GES, especially at 354 ng L-1, induced masculinization of female fish, indicated by the reduced body weight to length ratio and development of gonopodia (i.e. anal fins of male fish). Thus, the males showed less intimacy and mating interest towards the GES-exposed females, indicated by the reduced time spent on attending, following and mating behaviours. While oocyte development was seemingly unaffected by GES, spermatogonia were developed in the ovary. All the aforementioned masculinizing effects of GES were associated with the increased testosterone level and decreased estradiol level, driven by upregulating androgen receptor genes (Ar alpha and Ar beta). Overall, our findings suggest that progestins could undermine the reproductive potential of aquatic organisms and hence their persistence in the progestin-contaminated environment. (C) 2021 Elsevier B.V. All rights reserved.