CULTURE MEDIUM FOR ENTEROBACTERIA
CULTURE MEDIUM FOR ENTEROBACTERIA
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DOI：
10.1128/jb.119.3.736-747.1974
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发表时间：
1974-01-01
影响因子：
3.2
通讯作者：
SMITH, DF
中科院分区：
文献类型：
--
作者：
NEIDHARDT, FC;BLOCH, PL;SMITH, DF
一种新的肠杆菌基本培养基已经开发出来。它支持大肠杆菌和鼠伤寒沙门氏菌的生长，其速率与任何具有高磷酸盐浓度的传统培养基相当，但每种常量营养素（磷酸盐，硫酸盐和氮）的含量都足够低，可以进行同位素标记。缓冲能力由有机偶极离子吗啉代丙烷磺酸盐提供，其具有理想的pK（7.2）并且对生长没有明显的抑制作用。该培养基的目的是（i）提供化学组成的再现性，（ii）满足实验确定的细胞营养需求，（iii）避免主要离子种类的不必要过量，（iv）促进单个离子种类水平的调节，用于同位素标记和营养研究，（v）提供完整的微量营养素阵列，（vi）当碳和能源过量时，将特定离子设定为作物限制因子，和（vii）在培养基的制造和储存中提供最大的便利。
A new minimal medium for enterobacteria has been developed. It supports growth ofEscherichia coliandSalmonella typhimuriumat rates comparable to those of any of the traditional media that have high phosphate concentrations, but each of the macronutrients (phosphate, sulfate, and nitrogen) is present at a sufficiently low level to permit isotopic labeling. Buffering capacity is provided by an organic dipolar ion, morpholinopropane sulfonate, which has a desirable pK (7.2) and no apparent inhibitory effect on growth. The medium has been developed with the objectives of (i) providing reproducibility of chemical composition, (ii) meeting the experimentally determined nutritional needs of the cell, (iii) avoiding an unnecessary excess of the major ionic species, (iv) facilitating the adjustment of the levels of individual ionic species, both for isotopic labeling and for nutritional studies, (v) supplying a complete array of micronutrients, (vi) setting a particular ion as the crop-limiting factor when the carbon and energy source is in excess, and (vii) providing maximal convenience in the manufacture and storage of the medium.