PSEUDO HALF-KNOT FORMATION WITH RNA
PSEUDO HALF-KNOT FORMATION WITH RNA
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DOI：
10.1126/science.1502560
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发表时间：
1992-08-14
期刊：
影响因子：
56.9
通讯作者：
ZOUNES, M
中科院分区：
文献类型：
--
作者：
ECKER, DJ;VICKERS, TA;ZOUNES, M
伪半结可以通过不对称地将寡核苷酸与RNA发夹环结合而形成。该结合基序用于靶向人类免疫缺陷病毒TAR元件，这是一种重要的病毒RNA结构，是Tat的受体，Tat是主要的病毒反激活蛋白。与TAR结构的不同半部分互补的寡核苷酸比设计在发夹周围对称结合的分子具有更大的亲和力。伪半结形成的寡核苷酸改变了TAR的结构，从而破坏了TAR衍生肽的特异性识别和结合。这种一般结合基序可用于破坏调节性RNA发夹的结构。
A pseudo-half-knot can be formed by binding an oligonucleotide asymmetrically to an RNA hairpin loop. This binding motif was used to target the human immunodeficiency virus TAR element, an important viral RNA structure that is the receptor for Tat, the major viral transactivator protein. Oligonucleotides complementary to different halves of the TAR structure bound with greater affinity than molecules designed to bind symmetrically around the hairpin. The pseudo-half-knot-forming oligonucleotides altered the TAR structure so that specific recognition and binding of a Tat-derived peptide was disrupted. This general binding motif may be used to disrupt the structure of regulatory RNA hairpins.