The influence of omega-3 polyunsaturated fatty acids (omega-3 pufa) on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets.
The influence of omega-3 polyunsaturated fatty acids (omega-3 pufa) on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets.
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发表时间：
2003-07
影响因子：
0.4
通讯作者：
A. Bomba;R. Nemcová;S. Gancarčíková;R. Herich;J. Pistl;V. Révajová;Z. Jonecová;A. Bugarský;M. Levkut;R. Kaštel;M. Baran;G. Lazar;M. Hluchý;S. Marsálková;J. Pošivák
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作者：
A. Bomba;R. Nemcová;S. Gancarčíková;R. Herich;J. Pistl;V. Révajová;Z. Jonecová;A. Bugarský;M. Levkut;R. Kaštel;M. Baran;G. Lazar;M. Hluchý;S. Marsálková;J. Pošivák
在限生猪中研究了应用 omega-3 多不饱和脂肪酸 (omega-3 PUFA) 对副干酪乳杆菌肠道定植和细胞免疫的影响。多不饱和脂肪酸的施用对副干酪乳杆菌对无菌仔猪空肠粘膜的粘附产生积极影响。与对照组相比，实验组仔猪空肠粘膜上附着的副干酪乳杆菌数量增加了12%（5.10 log 10/cm2 vs. 4.55 log 10/cm2）。粘附在 28 日龄无菌仔猪回肠和结肠粘膜上的副干酪乳杆菌计数在 C 组中分别达到 4.45 和 5.05 log 10/cm2，在 E 组中分别达到 4.44 和 4.95 log 10/cm2。补充 Omega-3 PUFA 使中性粒细胞的吞噬活性在第 28 天增加了几乎 100%。生活以及 出生第 21 天无菌仔猪外周血中的淋巴细胞亚群 (CD8)。我们的结果表明，益生菌在肠道中的作用可能受到膳食多不饱和脂肪酸的调节。 PUFA对乳酸菌粘附的刺激作用可用于增强益生菌抑制消化道病原体的有效性。
The effect of application of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on intestinal colonization by Lactobacillus paracasei and on cellular immunity has been investigated in gnotobiotic pigs. The administration of polyunsaturated fatty acids positively affected the adhesion of Lactobacillus paracasei to the jejunal mucosa of gnotobiotic piglets. When compared to the control group, the number of Lactobacillus paracasei adhering to the jejunal mucosa was by 12% higher in piglets of the experimental group (5.10 log 10/cm2 vs. 4.55 log 10/cm2). The respective counts of Lactobacillus paracasei adhering to the ileal and colonic mucosa of 28 day old gnotobiotic piglets reached 4.45 and 5.05 log 10/cm2 in group C and 4.44 and 4.95 log 10/cm2 in group E. Omega-3 PUFA supplementation increased the phagocytic activity of neutrophils by almost 100% on day 28 of life as well as the subpopulations of lymphocytes (CD8) in the peripheral blood of germ-free piglets on day 21 of life. Our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA. The stimulatory effect of PUFA upon adhesion of lactobacilli could be used for enhancing the effectiveness of probiotics in inhibiting digestive tract pathogens.