Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine
Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine
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DOI：
10.1021/ac971383v
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发表时间：
1998-08-15
影响因子：
7.4
通讯作者：
Maibach, HI
中科院分区：
文献类型：
--
作者：
Gilman, SD;Gee, SJ;Maibach, HI
加速器质谱仪(AMS)已被应用于三嗪除草剂阿特拉津的C-14标记尿代谢物的检测，并将其分析性能与液体闪烁计数(LSC)进行了直接比较。给10名受试者皮下注射14C标记的阿特拉津超过24小时，并在7天内收集受试者的尿液，用AMS和LSC测定样品中的C-14浓度，范围为1.8fmoL/ml到4.3pmoL/ml。对于整个样本组的线性曲线图，这两种方法的数据具有0.998的相关系数。对于这些样品，加速器质谱仪提供了比LSC更高的浓度(2.2vs27fmoL/ml)和质量(5.5vs54000 amol)的检测限。AMS为低水平样品提供的数据的精确度为1.7%，AMS测量的日常重复性为3.9%。研究了限制这些样品的AMS检测限的因素以及提高这些限的方法。
Accelerator mass spectrometry (AMS) has been applied to the detection; of C-14-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting(LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period, Concentrations of C-14 in the samples have been determined by AMS and LSC and range from 1.8 fmol/ml to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/ml) and mass (5.5 vs 54000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%, Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.