Assessing Pressurized Liquid Extraction for the High-Throughput Extraction of Marine-Sponge-Derived Natural Products
Assessing Pressurized Liquid Extraction for the High-Throughput Extraction of Marine-Sponge-Derived Natural Products
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DOI：
10.1021/np900565a
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发表时间：
2010-03-01
影响因子：
5.1
通讯作者：
Crews, Phillip
中科院分区：
文献类型：
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作者：
Johnson, Tyler A.;Morgan, Micaela V. C.;Crews, Phillip
为了比较标准溶剂分配法(SSP)和加速溶剂萃取法(ASE)的有效性，进行了一系列的实验和评价。比较了两种方法得到的馏分的总收率、溶剂消耗量、处理时间和化学稳定性。选择了5种海绵进行加工和分析，其中包含12种结构不同的生物活性天然产物。使用比较的LC MS-ELSD对SSP和ASF产生的提取物进行化学降解评估。ASE装置的萃取效率(EE)平均为SSP法的3倍，而总萃取率(Tey)大致相当。此外，ASE方法处理每个样品只需要2小时，而SSR方法需要80小时，两种方法的LC MS-ELSD前提取物具有可比性。这些结果表明，ASE可以作为高效的高通量提取海洋生物的方法，以简化新的生物活性天然产物的发现。
In order to compare the utility of standard solvent partitioning (SSP) versus accelerated solvent extraction (ASE), a series of experiments were performed and evaluated. Overall yields, solvent consumption, processing time, and chemical stability of the Fractions obtained by both methods were compared. Five marine sponges were selected for processing and analysis containing 12 structurally distinct, bioactive natural products. Extracts generated using SSP and ASF were assessed for chemical degradation using, comparative LC MS-ELSD. The extraction efficiency (EE) of the ASE apparatus was 3 times greater than the SSP method on average, while the total extraction yields (TEY) were roughly equivalent. Furthermore, the ASE methodology required only 2 h to process each sample versus 80 h for SSR and the LC MS-ELSD front extracts of both methods appeared comparable. These results demonstrate that ASE can serve as in effective high-throughput methodology for extracting marine organisms to streamline the discovery of novel and bioactive natural products.