Cell cycle phase expansion in nitrogen-limited cultures of Saccharomyces cerevisiae.
Cell cycle phase expansion in nitrogen-limited cultures of Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.85.1.96
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发表时间：
1980-04
期刊：
影响因子：
--
通讯作者：
Fangman WL
中科院分区：
文献类型：
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作者：
Rivin CJ;Fangman WL
在芽殖酵母酿酒酵母中检查了细胞周期事件的时间和协调。全细胞放射自显影技术和延时摄影用于测量生长速率由氮源决定的细胞中S、G1和G2期的持续时间，以及细胞周期“开始”和芽出现的位置。观察到随着细胞周期长度的增加，G1、S和G2期经历了成比例的扩张，其中S期占据了细胞周期的中半部分。在每种生长条件下，起始点似乎对应于 G1 相/S 相边界。直到S期中期才出现芽。这些结果表明，当细胞周期长度发生变化时，穿过细胞周期所有阶段的速率可能会发生很大变化。当以不同速率生长的细胞在 G1 停止时，接下来的同步 S 期的持续时间是根据每个异步群体中 S 的长度所预期的。在 G1 期停滞后，从不良氮源转移到良好氮源的细胞以较好培养基的速率特征经历随后的 S 期，表明细胞在 G1 期并未进入特定持续时间的 S 期。
The time and coordination of cell cycle events were examined in the budding yeast Saccharomyces cerevisiae. Whole-cell autoradiographic techniques and time-lapse photography were used to measure the duration of the S, G1, and G2 phases, and the cell cycle positions of "start" and bud emergence, in cells whose growth rates were determined by the source of nitrogen. It was observed that the G1, S, and G2 phases underwent a proportional expansion with increasing cell cycle length, with the S phase occupying the middle half of the cell cycle. In each growth condition, start appeared to correspond to the G1 phase/S phase boundary. Bud emergence did not occur until mid S phase. These results show that the rate of transit through all phases of the cell cycle can vary considerably when cell cycle length changes. When cells growing at different rates were arrested in G1, the following synchronous S phase were of the duration expected from the length of S in each asynchronous population. Cells transferred from a poor nitrogen source to a good one after arrest in G1 went through the subsequent S phase at a rate characteristic of the better medium, indicating that cells are not committed in G1 to an S phase of a particular duration.