SITE-SPECIFIC RECOMBINATION BETWEEN HOMOLOGOUS CHROMOSOMES IN DROSOPHILA
SITE-SPECIFIC RECOMBINATION BETWEEN HOMOLOGOUS CHROMOSOMES IN DROSOPHILA
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DOI：
10.1126/science.2035025
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发表时间：
1991-05-17
期刊：
影响因子：
56.9
通讯作者：
GOLIC, KG
中科院分区：
文献类型：
--
作者：
GOLIC, KG
对一个细胞及其后代进行基因标记，从而使产生的细胞克隆能够与邻近细胞区分开来，这种能力有助于动物生物学和发育方面的研究。本文描述了一种通过位点特异性酵母重组酶在果蝇中诱导同源有丝分裂重组来产生克隆的方法。这种方法在基因组的预定位点诱导有丝分裂交换后会经常产生嵌合现象。
The ability to mark a cell and its descendants genetically so that the resulting cell clone can be distinguished from neighboring cells facilitates studies in animal biology and development. A method of generating clones by inducing homologous mitotic recombination in Drosophila with a site-specific yeast recombinase is described. This method allows for frequent mosaicism after mitotic exchange is induced at predefined sites in the genome.