RRM-RNA recognition: NMR or crystallography ... and new findings
RRM-RNA recognition: NMR or crystallography ... and new findings
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DOI：
10.1016/j.sbi.2012.11.006
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发表时间：
2013-02-01
影响因子：
6.8
通讯作者：
Allain, Frederic H-T
中科院分区：
文献类型：
--
作者：
Daubner, Gerrit M.;Clery, Antoine;Allain, Frederic H-T
要在分子水平上表征蛋白质-RNA识别，结构生物学已被证明是不可或缺的方法。蛋白质-RNA结构，特别是最丰富的RNA识别基序(RRM)，已经对RNA结合和特异性的机制有了详细和直接的见解。虽然这个蛋白质结构域具有非常保守的α-β折叠，但它可以识别大量不同的RNA序列和形状，并参与多种生物过程。在这里，我们报告了最近的单个和多个RRM-RNA结构，并指出了那些特征，为RRMS识别RNA的机制提供了新的见解。我们进一步概述了核磁共振光谱学和X射线结晶学方法的固有问题，并回顾了最近强调需要使用这两种方法来更快速和准确地确定结构的策略。
To characterize protein-RNA recognition at the molecular level, structural biology has turned out to be an indispensable approach. Detailed and direct insights into the mechanism of RNA binding and specificity have emerged from protein-RNA structures, especially from the most abundant RNA recognition motif (RRM). Although this protein domain has a very conserved alpha-beta fold, it can recognize a large number of different RNA sequences and shapes and can be involved in a multitude of biological processes. Here, we report on recent single and multiple RRM-RNA structures and point out those features that provide novel insights into the mechanism of RNA recognition by RRMs. We further outline inherent problems to both NMR spectroscopy and X-ray crystallography methods and review recent strategies that emphasize the need to use both methodologies for more rapid and accurate structure determinations.