Preferential transfer of 2-docosahexaenoyl-1-lysophosphatidylcholine through an in vitro blood-brain barrier over unesterified docosahexaenoic acid
Preferential transfer of 2-docosahexaenoyl-1-lysophosphatidylcholine through an in vitro blood-brain barrier over unesterified docosahexaenoic acid
复制标题
DOI：
10.1046/j.1471-4159.1999.0720338.x
复制
发表时间：
1999-01-01
影响因子：
4.7
通讯作者：
Lecerf, J
中科院分区：
文献类型：
--
作者：
Bernoud, N;Fenart, L;Lecerf, J
研究了未酯化的二十二碳六烯酸（DHA）或含溶血磷脂酰胆碱的DHA（lysoPC - DHA）通过血脑屏障体外模型的情况。该模型由置于星形胶质细胞培养介质上的脑毛细血管内皮细胞单层构成。细胞在无血清的介质中孵育4小时，然后将内皮细胞介质替换为含有标记的DHA或lysoPC - DHA的相同介质，并孵育2小时。测量细胞对DHA的摄取以及它向底层介质（当存在星形胶质细胞时为星形胶质细胞介质）的转移。当在孵育过程中保留预孵育的底层介质和星形胶质细胞时，lysoPC - DHA的通过量高于未酯化的DHA。当去除星形胶质细胞时，两种形式的通过量都下降。当预孵育的底层介质被新鲜介质替换时，未观察到对lysoPC - DHA的偏好，而当向底层介质中添加白蛋白时，这种偏好则逆转。在没有星形胶质细胞培养的脑内皮细胞培养2小时后也出现了lysoPC - DHA优先通过的情况，但在相同条件下培养和孵育的主动脉内皮细胞中未出现这种情况。总之，这些结果表明血脑屏障细胞释放了有利于DHA转移的成分，并对lysoPC - DHA表现出偏好。
The passage of either unesterified docosahexaenoic acid (DHA) or lysophosphatidylcholine-containing DHA (lysoPC-DHA) through an in vitro model of the blood-brain barrier was investigated. The model was constituted by a brain capillary endothelial cell monolayer set over the medium of an astrocyte culture. Cells were incubated for 4 h with a medium devoid of serum, then the endothelial cell medium was replaced by the same medium containing labeled DHA or lysoPC-DHA and incubations were performed for 2 h. DHA uptake by cells and its transfer to the lower medium (astrocyte medium when they were present) were measured. When the lower medium from preincubation and astrocytes were maintained during incubation, the passage of lysoPC-DHA was higher than that of unesterified DHA. The passage of both forms decreased when astrocytes were removed. The preference for lysoPC-DHA was not seen when the lower medium from preincubation was replaced by fresh medium, and was reversed when albumin was added to the lower medium. A preferential lysoPC-DHA passage also occurred after 2 h with brain endothelial cells cultured without astrocytes but not with aortic endothelial cells cultured and incubated under the same conditions. Altogether, these results suggest that the blood-brain barrier cells released components favoring the DHA transfer and exhibit a preference for lysoPC-DHA.