Regulation of neuronal and glial galectin-1 expression by peripheral and central axotomy of rat primary afferent neurons
Regulation of neuronal and glial galectin-1 expression by peripheral and central axotomy of rat primary afferent neurons
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DOI：
10.1016/j.expneurol.2005.04.004
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发表时间：
2005-09-01
影响因子：
5.3
通讯作者：
Ramer, MS
中科院分区：
文献类型：
--
作者：
McGraw, J;Gaudet, AD;Ramer, MS
半乳糖凝集素-1（Gal 1）是一种内源性表达的蛋白质，对于脊髓中初级感觉神经元及其突触连接的完整补体的胚胎发育非常重要。Gal 1还促进周围神经损伤后的轴突再生，但尚未研究初级传入神经元轴突切断对Gal 1的调节。在这里，我们通过免疫组织化学和原位杂交表明，Gal 1的表达差异调节周围神经损伤和背根切断术。周围神经损伤后，Gal 1阳性DRG神经元的比例增加。大直径DRG神经元免疫阳性Gal 1的比例的增加是由增加的免疫反应在背角，其中Gal 1阳性终端通常仅限于板层I和II的深度增加。背根切断术并没有影响的比例，含Gal 1 mRNA或蛋白质的神经元，但没有耗尽同侧背角的Gal 1免疫反应，表明它是由背根轴突集中运输。背根切断术也导致Gal 1 mRNA的增加，神经周围的PNS-CNS接口（可能在施瓦尔细胞和/或巨噬细胞），并在较小程度上在去传入脊髓区域进行沃勒变性。后者的增加是显着的背柱和沿着以前的轨迹有髓传入到更深的背角。这些结果表明Gal 1的神经元和神经胶质表达与再生成功密切相关。因此，Gal 1的差异表达模式的外周轴突和背根切断术后，内源性Gal 1可能是一个重要的因素损伤轴突的再生反应。(c)2005年爱思唯尔公司All rights reserved.
Galectin-1 (Gal1) is an endogenously-expressed protein important for the embryonic development of the full complement of primary sensory neurons and their synaptic connections in the spinal cord. Gal1 also promotes axonal regeneration following peripheral nerve injury, but the regulation of Gal1 by axotomy in primary afferent neurons has not yet been examined. Here, we show by immunohistochemistry and in situ hybridization that Gal1 expression is differentially regulated by peripheral nerve injury and by dorsal rhizotomy. Following peripheral nerve injury, the proportion of Gal1-positive DRG neurons was increased. An increase in the proportion of large-diameter DRG neurons immunopositive for Gal1 was paralleled by an increase in the depth of immunoreactivity in the dorsal horn, where Gal1-positive terminals are normally restricted to laminae I and II. Dorsal rhizotomy did not affect the proportions of neurons containing Gal1 mRNA or protein, but did deplete the ipsilateral dorsal horn of Gal1 immunoreactivity, indicating that it is transported centrally by dorsal root axons. Dorsal rhizotomy also resulted in an increase in Gal1 mRNA the nerve peripheral to the PNS-CNS interface (likely within Schwarm cells and/or macrophages), and to a lesser extent within deafferented spinal cord regions undergoing Wallerian degeneration. This latter increase was notable in the dorsal columns and along the prior trajectories of myelinated afferents into the deeper dorsal horn. These results show that neuronal and glial expressions of Gal1 are tightly correlated with regenerative success. Thus, the differential expression pattern of Gal1 following peripheral axotomy and dorsal rhizotomy suggests that endogenous Gal1 may be a factor important to the regenerative response of injured axons. (c) 2005 Elsevier Inc. All rights reserved.