Comparison of aerobic denitrifying activity among three cultural species with various carbon sources.
Comparison of aerobic denitrifying activity among three cultural species with various carbon sources.
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DOI：
10.2166/wst.2004.0477
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发表时间：
2004-10
期刊：
影响因子：
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通讯作者：
Y. Otani;K. Hasegawa;K. Hanaki
中科院分区：
文献类型：
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作者：
Y. Otani;K. Hasegawa;K. Hanaki
从硝酸盐去除效率和有机物利用率的角度比较了粪产碱杆菌、产气脱氮微生物和泛养副球菌三种好氧反硝化菌的能力。首先，研究了碳源的影响。尽管在好氧条件下所有菌株都观察到硝酸盐还原，但碳源的变化极大地影响了反硝化能力。就P. pantotropicus而言，在乙酸钠或亮氨酸作为碳源的情况下，硝酸盐和亚硝酸盐在三天内被完全去除。对于粪曲霉，仅当添加乙酸钠或乙醇时才观察到充分的硝酸盐去除。 P. pantotropicus 和 A. faecalis 显示出比 M. aerodenitrificans 更高的硝酸盐去除能力。因此，选择 P. pantotropicus 来研究碳浓度和重复添加的影响。使用乙酸钠作为唯一的碳源。当碳浓度低于500mgC/L时，硝酸盐不被还原。然而，当反复添加碳源时，细菌光密度达到1.0以上后，硝酸盐降低至100mgC/L以下。该结果表明足够高水平的细菌密度对于表达好氧反硝化活性是必要的。
Abilities of three aerobic denitrifiers such as Alcaligenes faecalis, Microvirgula aerodenitrificans and Paracoccus pantotrophus were compared from the viewpoints of nitrate removal efficiency and organic matter utilization. First, the effect of carbon source was investigated. Although nitrate reduction was observed in all strains under aerobic conditions, a change of carbon source considerably affected the denitrification ability. In the case of P. pantotrophus, nitrate and nitrite were completely removed in three days under sodium acetate or leucine as a carbon source. In the case of A. faecalis, sufficient nitrate removal was observed only when sodium acetate or ethanol was added. P. pantotrophus and A. faecalis showed a higher ability of nitrate removal than that of M. aerodenitrificans. Therefore, P. pantotrophus was selected in order to investigate the effects of concentration and repetitive addition of carbon. Sodium acetate was used as a sole carbon source. Nitrate was not reduced when the carbon concentration was below 500 mgC/L. However, when carbon source was added repeatedly, nitrate was reduced under 100 mgC/L after the optical density of the bacterium reached above 1.0. This result indicated that a high enough level of bacterial density was necessary to express aerobic denitrification activity.