Significance of Tsukushi in lung cancer.
Significance of Tsukushi in lung cancer.
复制标题
DOI：
10.1016/j.lungcan.2019.03.024
复制
发表时间：
2019-05
期刊：
影响因子：
5.3
通讯作者：
Tatsuya Yamada;K. Ohta;Y. Motooka;K. Fujino;S. Kudoh;Yuki Tenjin;Younosuke Sato;Akira Matsuo;Koei Ikeda;Makoto Suzuki;Takaaki Ito
中科院分区：
文献类型：
--
作者：
Tatsuya Yamada;K. Ohta;Y. Motooka;K. Fujino;S. Kudoh;Yuki Tenjin;Younosuke Sato;Akira Matsuo;Koei Ikeda;Makoto Suzuki;Takaaki Ito
目的Tsukushi（TSK）是小分子富含亮氨酸重复蛋白聚糖（SLRP）家族的成员，在发育过程中与信号分子相互作用，发挥多功能作用。然而，TSK在癌症中的作用仍然未知。为探讨TSK在肺癌中的生物学意义，采用免疫组化和逆转录聚合酶链反应（PCR）方法检测肺癌组织中TSK的表达。然后，在TSK表达水平改变的人肺腺癌细胞系H1975中进行RNA序列分析、基因本体分析、定量实时PCR、蛋白质印迹、细胞计数测定、侵袭测定和异种移植研究，以研究其生物学作用，结果TSK在肺癌组织和细胞系中高表达。H1975中TSK表达水平的改变导致EMT相关分子的变化，包括钙粘蛋白-1、蜗牛家族转录抑制因子1、蜗牛家族转录抑制因子2和波形蛋白。细胞计数和异种移植实验结果表明，TSK促进细胞增殖.ConclusionsIn肺癌细胞中，TSK比其他SLRPs家族成员表达更高，并调节EMT和增殖。因此，TSK可能是多种途径的关键协调者，也是肺癌微环境中的重要结构元件。
ObjectivesTsukushi (TSK), a member of the small leucine-rich repeat proteoglycan (SLRP) family, plays multifunctional roles by interacting with signaling molecules during development. However, the role of TSK in cancer remains unknown. The aim of the present study was to investigate the biological significance of TSK in lung cancer.Materials and methodsImmunohistochemistry of lung cancer tissues and reverse transcription polymerase chain reaction (PCR) of lung cancer cell lines were carried out to detect TSK. Then, RNA sequence analysis, Gene Ontology analysis, quantitative real-time PCR, western blotting, cell counting assay, invasion assays, and xenograft studies were done in a human lung adenocarcinoma cell line, H1975 with modification of TSK expression levels, in order to investigate its biological roles, in particular epithelial–mesenchymal transition (EMT) and proliferation.ResultsTSK was found to be highly expressed in lung cancer tissues and cell lines. Modification of TSK expression levels in H1975 resulted in changes in molecules related to EMT, including cadherin-1, snail family transcriptional repressor 1, snail family transcriptional repressor 2, and vimentin. The results of cell counting and xenograft assays showed that TSK promotes cell proliferation.ConclusionsIn lung cancer cells, TSK is expressed more highly than the other SLRPs family members, and regulates the EMT and proliferation. Thus, TSK may be a key coordinator of multiple pathways and an important structural element in the lung cancer microenvironment.