Integrating transcriptome, co-expression and QTL-seq analysis reveals that primary root growth in maize is regulated via flavonoid biosynthesis and auxin signal transduction
Integrating transcriptome, co-expression and QTL-seq analysis reveals that primary root growth in maize is regulated via flavonoid biosynthesis and auxin signal transduction
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整合转录组、共表达和 QTL-seq 分析揭示玉米初生根生长是通过类黄酮生物合成和生长素信号转导调节的
DOI：
10.1093/jxb/erab177
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发表时间：
2021
影响因子：
6.9
通讯作者：
Li Pengcheng
中科院分区：
文献类型：
--
作者：
Wang Yunyun;Sun Hui;Wang Houmiao;Yang Xiaoyi;Xu Yang;Yang Zefeng;Xu Chenwu;Li Pengcheng
初生根对早期幼苗的生长和存活至关重要。为了了解主根发育的分子机制，我们进行了动态转录组分析的两个玉米（玉米）自交系对比主根长度在9个时间点超过12天的时间。共检测到18702个基因在两株系间或不同时间点的差异表达。基因富集、植物激素含量测定和代谢组学分析表明，生长素的生物合成和信号转导以及苯丙烷和类黄酮的生物合成途径与根的发育有关。共表达网络分析表明，8个模块与线/阶段，以及初级或侧根长度。在根相关模块中，类黄酮代谢伴随生长素的合成和信号转导，构成了一个复杂的基因调控网络。通过共表达网络分析、QTL-seq分析和功能注释，筛选出2个与生长素信号传导和类黄酮合成相关的候选基因（无根冠和种子根基因rtcs和Zm 00001 d 012781）。这些结果加深了我们对控制玉米主根和侧根长度发育的调控网络的理解，为玉米根系性状的改良提供了有价值的遗传资源。
The primary root is critical for early seedling growth and survival. To understand the molecular mechanisms governing primary root development, we performed a dynamic transcriptome analysis of two maize (Zea mays) inbred lines with contrasting primary root length at nine time points over a 12-day period. A total of 18 702 genes were differentially expressed between two lines or different time points. Gene enrichment, phytohormone content determination, and metabolomics analysis showed that auxin biosynthesis and signal transduction, as well as the phenylpropanoid and flavonoid biosynthesis pathways, were associated with root development. Co-expression network analysis revealed that eight modules were associated with lines/stages, as well as primary or lateral root length. In root-related modules, flavonoid metabolism accompanied by auxin biosynthesis and signal transduction constituted a complex gene regulatory network during primary root development. Two candidate genes (rootless concerning crown and seminal roots,rtcsandZm00001d012781) involved in auxin signaling and flavonoid biosynthesis were identified by co-expression network analysis, QTL-seq and functional annotation. These results increase our understanding of the regulatory network controlling the development of primary and lateral root length, and provide a valuable genetic resource for improvement of root performance in maize.