Evaluation of genital sites and sampling techniques for detection of human papillomavirus DNA in men
Evaluation of genital sites and sampling techniques for detection of human papillomavirus DNA in men
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DOI：
10.1086/381395
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发表时间：
2004-02-15
影响因子：
6.4
通讯作者：
Koutsky, LA
中科院分区：
文献类型：
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作者：
Weaver, BA;Feng, QH;Koutsky, LA
为了评估检测男性生殖器人乳头瘤病毒 (HPV) DNA 的方法，从性传播疾病诊所就诊的 10 名男性连续 3 组获取样本，方法是：(1) 单独使用盐水润湿的涤纶棉签，(2) 盐水润湿的细胞刷，或 (3) 金刚砂纸 (600A-grit Wetordry Tri-M-ite; 3M) 摩擦，然后用盐水润湿的涤纶拭子。通过使用基于聚合酶链反应的检测方法，发现 45% 的金刚砂纸样本的 β-珠蛋白呈阳性，而仅拭子样本和细胞刷样本的这一比例分别为 23% 和 0%。随后，使用金刚砂纸和盐水润湿的涤纶拭子从 318 名男性大学生身上获取阴茎干、龟头、包皮和阴囊样本。还获取了尿液样本。在检测的 1323 个样本中，有 1288 个（97%）被发现 β-珠蛋白呈阳性。在 104 名男性 (33%) 的样本中检测到 HPV DNA：24% 来自阴茎干，16% 来自龟头，28% 来自包皮，17% 来自阴囊，6% 来自尿液。接受包皮环切术和未接受包皮环切术的男性的 HPV 患病率相似。多个部位检测增加了检测到 HPV DNA 的男性数量。
To evaluate methods for detection of genital human papillomavirus (HPV) DNA in men, samples were obtained from 3 consecutive groups of 10 men attending a sexually transmitted disease clinic by use of (1) a saline-wetted Dacron swab alone, (2) a saline-wetted cytobrush, or (3) emery paper (600A-grit Wetordry Tri-M-ite; 3M) abrasion followed by a saline-wetted Dacron swab. By use of a polymerase chain reaction-based assay, 45% of emery-paper samples were found to be positive for beta-globin, compared with 23% of swab-alone and 0% of cytobrush samples. Subsequently, emery paper and saline-wetted Dacron swabs were used to obtain penile shaft, glans, foreskin, and scrotum samples from 318 male university students. Urine samples were also obtained. Of 1323 samples tested, 1288 (97%) were found to be positive for beta-globin. HPV DNA was detected in samples from 104 men (33%): 24% from the penile shaft, 16% from the glans, 28% from the foreskin, 17% from the scrotum, and 6% in urine. The HPV prevalence was similar for circumcised and uncircumcised men. Testing multiple sites increased the number of men for whom HPV DNA was detected.