Antimicrobial effect of acidified nitrite on gut pathogens: Importance of dietary nitrate in host defense
Antimicrobial effect of acidified nitrite on gut pathogens: Importance of dietary nitrate in host defense
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DOI：
10.1128/aac.40.6.1422
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发表时间：
1996-06-01
影响因子：
4.9
通讯作者：
Leifert, C
中科院分区：
文献类型：
--
作者：
Dykhuizen, RS;Frazer, R;Leifert, C
从饮食中摄入硝酸盐会产生唾液亚硝酸盐，在胃中酸化，产生许多氮的活性中间体，其中包括潜在致癌的N-亚硝胺。然而，酸化的亚硝酸盐也具有抗菌活性，这与一氧化氮的形成一致。本研究考察了酸化亚硝酸盐对沙门氏菌、鼠伤寒沙门氏菌、小肠结肠炎耶尔森氏菌、宋内氏志贺菌和大肠杆菌O 157的体外抗菌作用。一阶回归图显示，在30 min、2 h、24 h时，各抗菌活性差异均显著（P < 0.001）。enterocolitica> S.添加SCN-可增强抗菌活性（配对t检验，P <0.001），而添加Cl-对抗菌活性无显著影响。
Dietary intake of nitrate generates salivary nitrite, which is acidified in the stomach, leading to a number of reactive intermediates of nitrogen, among which are the potentially carcinogenic N-nitrosamines. Acidified nitrite, however, also has antimicrobial activity which coincides with the formation of nitric: oxide. The present study examines the antimicrobial effect in vitro of acidified nitrite on Salmonella enteritidis, Salmonella typhimurium, Yersinia enterocolitica, Shigella sonnei, and Escherichia coli O157, First-order regression plots showed a linear inverse relationship of log-transformed proton and nitrite concentrations with MICs and MBCs after 30 min, 2 h, and 24 h of exposure (P < 0.001 for all antibacterial activities), Susceptibility to the acidified nitrate solutions ranked as follows: Y. enterocolitica > S, enteritidis > S. typhimurium = Shigella sonnei > E, coli O157 (P < 0.05), Addition of SCN-, but not that of Cl-, increased the antibacterial activity (paired t testing, P < 0.001), Generation of salivary nitrite from dietary nitrate may provide significant protection against gut pathogens in humans.