In vitro synthesis of superoxide dismutases of rat liver.
In vitro synthesis of superoxide dismutases of rat liver.
复制标题
大鼠肝脏超氧化物歧化酶的体外合成。
DOI：
10.1016/0006-291x(85)91407-x
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
K. Toyoshi
中科院分区：
文献类型：
--
作者：
K. Hirano;M. Fukuta;T. Adachi;K. Hayashi;M. Sugiura;Y. Mori;K. Toyoshi
研究了铜、锌超氧化物歧化酶（Cu，Zn-SOD）和锰超氧化物歧化酶（Mn-SOD）的体外合成。Cu，Zn-SOD和Mn-SOD均优先由游离多核糖体合成。Mn-SOD合成为大前体（26，000道尔顿），其通过与大鼠肝线粒体组分体外孵育加工成成熟大小（22，500道尔顿）。另一方面，以Cu，Zn-SOD为原料，合成了成熟的浆料产品。结果表明，体外合成的Cu，Zn-SOD和Mn-SOD分别占游离多核糖体总翻译产物的0.018%和0.016%。
The syntheses of copper, zinc-superoxide dismutase (Cu, Zn-SOD) and manganese-superoxide dismutase (Mn-SOD) in vitro were studied. Both Cu, Zn-SOD and Mn-SOD were preferentially synthesized by free polysomes. Mn-SOD was synthesized as a large precursor (26,000 daltons), which was processed to the mature size (22,500 daltons) by in vitro incubation with a rat liver mitochondrial fraction. On the other hand, Cu, Zn-SOD was synthesized as the mature size product. It was shown that Cu, Zn-SOD and Mn-SOD synthesized in vitro represented 0.018% and 0.016% of the total translation products of free polysomes, respectively.