Fibronectin in early amphibian embryos
Fibronectin in early amphibian embryos
复制标题
早期两栖动物胚胎中的纤连蛋白
DOI：
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发表时间：
1983
影响因子：
3.6
通讯作者：
T. Darribère
中科院分区：
文献类型：
--
作者：
J. Boucaut;T. Darribère
摘要利用针对两栖动物 FN 的抗体，研究了早期两栖动物胚胎（Ambystoma mexicanum、Pleurodeles waltlii）中纤连蛋白（FN）的三维组织。对整体标本进行免疫荧光标记。结果表明，在原肠胚形成之前，在中胚层细胞迁移之前，囊胚腔顶部下方存在由吻合的 FN 原纤维组成的广泛细胞外网络。最初，FN-原纤维在外胚层细胞的内表面（早期囊胚阶段）呈放射状发育，并在那里汇合以形成原纤维网络。在囊胚晚期，囊胚腔顶部的整个表面（假定的外胚层和中胚层）被富含 FN 的细胞外基质覆盖。随着原肠胚形成的进行，迁移的中胚层细胞与 FN 纤维网络相互作用。结果表明，含有 FN 的细胞外基质在介导原肠胚中中胚层细胞-基质接触方面发挥着重要作用。在胚胎的其他部分没有发现FN-原纤维。
SummaryThe three-dimensional organisation of fibronectin (FN) in early amphibian embryos (Ambystoma mexicanum, Pleurodeles waltlii) was studied with the use of antibodies against amphibian-FN. Immunofluorescence labelling was performed on whole-mount specimens. It was shown that before gastrulation an extensive extracellular network consisting of anastomosed FN-fibrils underlies the roof of the blastocoel prior to the migration of mesodermal cells. Initially, FN-fibrils develop radially on the inner surface of ectodermal cells (early blastula stage) and become confluent there to elaborate a fibrillar network. During the late blastula stage the entire surface of the blastocoel roof (presumptive ectoderm and mesoderm) is covered by the FN-rich extracellular matrix. As gastrulation proceeds, the migrating mesodermal cells interact with the FN-fibrillar network. The results suggest that the FN-containing extracellular matrix plays an important role in mediating mesodermal cell-substratum contacts in gastrulating embryos. No FN-fibrils were found in other parts of the embryo.