Impaired Gas Bladder Inflation in Zebrafish Exposed to a Novel Heterocyclic Brominated Flame Retardant Tris(2,3-dibromopropyl) Isocyanurate
Impaired Gas Bladder Inflation in Zebrafish Exposed to a Novel Heterocyclic Brominated Flame Retardant Tris(2,3-dibromopropyl) Isocyanurate
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DOI：
10.1021/es202420g
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发表时间：
2011-11-15
影响因子：
11.4
通讯作者：
Jiang, Guibin
中科院分区：
文献类型：
--
作者：
Li, Juan;Liang, Yong;Jiang, Guibin
硬骨鱼的气囊是一个充满气体的内部器官，负责气体交换和控制浮力。在这里，我们报告了一种新兴的杂环溴化阻燃剂，三（2，3-二溴丙基）异氰脲酸酯（TBC），导致缺陷的斑马鱼幼虫的气囊膨胀。这可能会导致运动能力受损，最终导致死亡。暴露于斑马鱼胚胎显示，TBC对受精后72-96 h的幼虫有最显著的影响，这与气囊首次充气的时间相吻合。斑马鱼早期气囊发育的关键因素保持在正常水平，这表明TBC在不破坏早期器官发生的情况下引起气囊充气缺陷。但经TBC处理后，气囊的超微结构发生改变：胞质小泡电子密度改变，线粒体受损。我们推断TBC导致线粒体损伤，影响粘液样物质的分泌，导致气囊充气缺陷。我们首次报道了膀胱可能是TBC的主要靶器官，并且应该将膀胱的评估包括在斑马鱼胚胎的毒性测试方案中。
The teleost gas bladder is a gas-filled internal organ that processes gas exchange and controls buoyancy. Here we report that an emerging heterocyclic brominated flame retardant, tris(2,3-dibromopropyl) isocyanurate (TBC), causes defects in the inflation of the gas bladder of zebrafish larvae. This could cause impaired motility, which can ultimately lead to their death. Exposure to zebrafish embryos revealed that TBC had the most significant influence on the larvae at 72-96 h postfertilization, which coincided with the time that the gas bladder first inflates. Critical factors involved in early zebrafish gas bladder development remained at normal levels, which indicated that TBC caused defects in the inflation of the gas bladder without disrupting early organogenesis. However, the ultrastructure of the gas bladder was altered in the TBC-treated groups: the electron density of cytoplasmic vesicles was changed and the mitochondria were damaged. We deduce that TBC causes damage to mitochondria that influences the secretion of mucus-like material, resulting in defects in gas bladder inflation. For the first time, we report that the gas bladder could be a primary target organ for TBC, and assessment of the gas bladder should be included in toxicity testing protocols of zebrafish embryos.