Comparative analysis of gastrointestinal microbiota between normal and caudal-related homeobox 2 (cdx2) transgenic mice.
Comparative analysis of gastrointestinal microbiota between normal and caudal-related homeobox 2 (cdx2) transgenic mice.
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DOI：
10.5217/ir.2015.13.1.39
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发表时间：
2015-01
影响因子：
4.9
通讯作者：
Sugano K
中科院分区：
文献类型：
--
作者：
Sakamoto H;Asahara T;Chonan O;Yuki N;Mutoh H;Hayashi S;Yamamoto H;Sugano K
尾侧相关同源盒2 （Cdx2）在人肠化生黏膜中表达，并在Cdx2转基因小鼠胃中诱导肠化生黏膜。萎缩性胃炎和肠化生通常会导致胃胃炎，这使胃容易细菌过度生长。在本研究中，我们使用定量逆转录聚合酶链反应（qRT-PCR）测定了正常小鼠和Cdx2转基因小鼠肠道微生物群的差异。分别处死正常小鼠12只（对照）和Cdx2转基因小鼠12只，收集胃、空肠、回肠、盲肠和结肠黏膜及粪便。为了定量细菌微生物群，我们使用real-time qRTPCR技术，采用16S rRNA基因靶向，物种特异性引物。Cdx2转基因小鼠胃、空肠、回肠、盲肠和结肠黏膜的细菌总数均显著高于正常小鼠。在Cdx2转基因小鼠胃中检测到脆弱拟杆菌组和普雷沃氏菌，但在正常小鼠胃中未检测到。此外，在Cdx2转基因小鼠的空肠和回肠中均未检测到球芽梭菌群、瘦梭菌亚群、脆弱拟杆菌群和普雷沃氏菌，但在正常小鼠的空肠和回肠中均未检测到。正常小鼠的粪便菌群与Cdx2转基因小鼠相似。我们的研究结果显示，正常小鼠和Cdx2转基因小鼠肠道菌群组成存在差异，这可能是由于Cdx2转基因小鼠胃胃酸和肠化生的发生引起的。
Caudal-related homeobox 2 (Cdx2) is expressed in the human intestinal metaplastic mucosa and induces intestinal metaplastic mucosa in the Cdx2 transgenic mouse stomach. Atrophic gastritis and intestinal metaplasia commonly lead to gastric achlorhydria, which predisposes the stomach to bacterial overgrowth. In the present study, we determined the differences in gut microbiota between normal and Cdx2 transgenic mice, using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Twelve normal (control) and 12 Cdx2 transgenic mice were sacrificed, and the gastric, jejunal, ileac, cecal and colonic mucosa, and feces were collected. To quantitate bacterial microbiota, we used real-time qRTPCR with 16S rRNA gene-targeted, species-specific primers. The total numbers of bacteria in the gastric, jejunal, ileac, cecal, and colonic mucosa of the Cdx2 transgenic mice were significantly higher than those of the normal mice. The Bacteroides fragilis group and also Prevotella were not detected in the stomach of the normal mice, although they were detected in the Cdx2 transgenic mice. Moreover, the Clostridium coccoides group, Clostridium leptum subgroup, Bacteroides fragilis group, and Prevotella were not detected in the jejunum or ileum of the normal mice, although they were detected in the Cdx2 transgenic mice. The fecal microbiota of the normal mice was similar to that of the Cdx2 transgenic mice. Our results showed the differences in composition of gut microbiota between normal and Cdx2 transgenic mice, which may be caused by the development of gastric achlorhydria and intestinal metaplasia in Cdx2 transgenic mice.