Tissue immunoassay for 19F-tagged 5-hydroxytryptophan.
Tissue immunoassay for 19F-tagged 5-hydroxytryptophan.
复制标题
19F 标记的 5-羟色氨酸的组织免疫测定。
DOI：
10.1081/ias-120025771
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Guo,Congyuan
中科院分区：
文献类型：
--
作者：
Dingman,Sherry;Hurlburt,Lara;Thomas,Rhys;Guo,Congyuan
合成了一种新的磁共振工具，1-6-七氟丁酰-5-羟基色氨酸，并使用先前开发的全氟烷基部分的抗体进行了研究。为了用作显像剂，该化合物必须穿过血脑屏障，然后浓缩在血清素能神经元的囊泡中，以便积累足够的量以进行体内检测。将新型显像化合物在卵内给予家养雏鸡（Gallus Domestics），以研究该化合物在该模型生物中的生物利用度和摄取动态。典型的免疫测定方法无效，因此开发了一种新技术，将胺和氨基酸结合到酸功能化比色皿的壁上。第一项研究证实，神经组织和肝脏组织中存在的标签数量高于心脏组织中的标签数量。第二项研究调查了区域差异，中脑比额叶样本含有更多的标记化合物，而额叶样本比枕叶或小脑样本含有更多的标记化合物。这些研究表明该化合物遵循内源性血清素的途径。第三项研究调查了新型化合物的吸收动力学。注射孵化第 14 天的鸡蛋三天后，大脑中标记分子的浓度达到最大，表明它在体内生物累积。这种用于检测组织样本中新型化合物的新免疫测定技术表现出良好的重复性。
A new tool for magnetic resonance,l-6-heptafluorobutyryl-5-hydroxytryptophan, was synthesized and investigated using an antibody to perfluoroalkyl moieties developed previously. To be useful as an imaging agent, the compound must cross the blood brain barrier and then be concentrated in vesicles in serotonergic neurons in order to accumulate in sufficient quantity for in vivo detection to be possible. The novel imaging compound was administered in ova to domestic chicks (Gallus domestics) to investigate the bioavailability and uptake dynamics of the compound in this model organism. Typical immunoassay methods were ineffective, so a new technique was developed which binds amines and amino acids to the walls of acid-functionalized cuvettes. The first study established the presence of higher quantities of the tags in neural and liver tissue than in heart tissue. A second study investigated regional differences, with the midbrain containing more tagged compounds than the frontal lobe sample, and the frontal lobe sample containing more than the occipital or cerebellum samples. These studies demonstrate that the compound follows the pathway of endogenous serotonin. A third study investigated uptake dynamics of the novel compound. Maximum concentration of the tagged molecule in the brain was achieved three days after injecting Incubation Day 14 eggs, suggesting that it bioaccumulates in vivo. This new immunoassay technique used to detect the novel compound in tissue samples demonstrated good repeatability.