CLASSES OF ANABAENA-VARIABILIS MUTANTS WITH OXYGEN-SENSITIVE NITROGENASE ACTIVITY

CLASSES OF ANABAENA-VARIABILIS MUTANTS WITH OXYGEN-SENSITIVE NITROGENASE ACTIVITY
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DOI:
10.1128/jb.136.2.688-692.1978
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发表时间:
1978-01-01
影响因子:
3.2
通讯作者:
WOLK, CP
WOLK, CP
中科院分区:
生物学3区
文献类型:
--
作者:
HAURY, JF;WOLK, CP

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异型囊包膜糖脂缺乏的变异单胞杆菌突变体在有氧测定时没有或极低的氮酶活性。能够在N2上有氧生长的还原剂增加了这些糖脂的数量。在需要固定氮才能在空气中生长并具有正常糖脂补充的突变体中,一个在低氧张力下表现出高的氮酶活性。另外三种仅在二亚硝酸盐存在时才表现出高的氮酶活性,因此在电子转移方面受到损害。
Mutants of A. variabilis deficient in the envelope glycolipids of heterocysts have no or very low nitrogenase activity when assayed aerobically. Revertants capable of aerobic growth on N2 have increased quantities of these glycolipids. Among mutants which require fixed N for growth in air and which have a normal complement of glycolipids, one expresses high nitrogenase activity at low O2 tension. Three others show high nitrogenase activity only in the presence of dithionite and are therefore impaired in electron transfer.