Genome-wide comparison of the GRAS protein family in eight Rosaceae species and GRAS gene expression analysis in Chinese white pear (Pyrus bretschneideri Rehder)
Genome-wide comparison of the GRAS protein family in eight Rosaceae species and GRAS gene expression analysis in Chinese white pear (Pyrus bretschneideri Rehder)
复制标题
八种蔷薇科植物 GRAS 蛋白家族的全基因组比较及白梨 (Pyrus bretschneideri Rehder) 的 GRAS 基因表达分析
DOI：
10.1080/01140671.2021.1936081
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发表时间：
2021-06-03
影响因子：
1.3
通讯作者：
Wu, Jun
中科院分区：
文献类型：
--
作者：
Chang, Wenjing;Sun, Manyi;Wu, Jun
摘要GRAS蛋白家族是一类植物特异性转录因子家族，在植物发育和花青素合成中具有重要的调控作用。本研究对蔷薇科植物GRAS蛋白家族基因进行了全面的鉴定和比较。在这里，535个GRAS基因从8种蔷薇科植物中筛选。系统发育分析表明，全基因组重复（WGD）事件和分散重复事件是促进GRAS蛋白家族在这些蔷薇科植物中扩展的主要机制。此外，不同重复事件引起的选择压力表明，GRAS基因在进化过程中主要处于纯化选择状态，说明强功能限制影响了GRAS基因的进化。基因表达谱分析和qRT-PCR分析表明，GRAS基因广泛参与梨的发育过程，且表达具有组织特异性。进一步分析表明，Pbr 010864.1和Pbr 024549.1可能是梨花色素苷合成过程中的两个重要候选基因。我们的研究是第一个在梨和其他蔷薇科物种的GRAS基因进行全面分析。这些结果为理解GRAS基因的进化过程和梨果实品质的改良提供了有益的信息。
ABSTRACT The GRAS protein family encodes a family of plant-specific transcription factors that have diverse and significant functions in regulating plant development and anthocyanin biosynthesis. Our study represents the comprehensive identification and comparison of GRAS protein family genes in the Rosaceae. Here, 535 GRAS genes were screened from eight Rosaceae species. Phylogenetic analysis revealed that whole-genome duplication (WGD) events and dispersed duplication events were the major mechanisms promoting the expansion of the GRAS protein family in these Rosaceae species. Furthermore, selection pressure caused by different duplication events indicates that GRAS genes were mainly under purifying selection during the evolution process, suggesting that strong functional restriction affected the evolution of GRAS genes. Analysis of gene expression patterns and qRT-PCR assays showed that GRAS genes were widely participating in the development of pear and that expression could be tissue-specific. In particular, further analysis indicated that Pbr010864.1 and Pbr024549.1 might be two important candidate genes during anthocyanin biosynthesis in pear. Our study is the first to perform a comprehensive analysis of the GRAS genes in pear and other Rosaceae species. These results presented here provide profitable information for understanding the process of the GRAS genes evolution and the possible improvement of pear fruit quality.