Partial characterization of immunoreactive substance P in the rat pituitary gland.
Partial characterization of immunoreactive substance P in the rat pituitary gland.
复制标题
大鼠垂体中免疫反应物质 P 的部分表征。
DOI：
10.1016/0024-3205(84)90594-0
复制
发表时间：
1984
期刊：
影响因子：
6.1
通讯作者：
McCann,SM
中科院分区：
文献类型：
--
作者：
DePalatis,LR;Khorram,O;Ho,RH;Negro-Vilar,A;McCann,SM
采用两种不同的羧基末端定向抗P物质（SP）血清（R-1C和R-6G）来表征成年雄性大鼠垂体前叶（AP）和垂体后叶（PP）乙酸提取物中的免疫反应性SP （I-SP）。R-1C和R-6G测定的I-SP组织浓度具有可比性。I-SP含量分别为600 ~ 1150 pg/AP和25 ~ 52 pg/PP。AP提取物中未检测到i -黄体生成素释放激素和i -生长抑素（I-SOM），但PP提取物中含有相当于325-785 pg的I-SOM/腺体。连续稀释AP和PP提取物后，两种SP抗血清的位移曲线与各自合成的SP标准和下丘脑提取物的位移曲线平行。在Sephadex G-25色谱柱上对AP和PP提取物进行凝胶过滤，得到的I-SP峰与合成SP和下丘脑I-SP相同。然而，AP I-SP剖面也显示了在空隙体积和主I-SP峰之间的侧峰迁移。当用6.0 M盐酸胍（一种强变性剂）洗脱时，AP提取物中的两种免疫反应物质都没有被消除。配对前半下垂在56 mM K+浓度下体外孵育30分钟，显著(p<。0001)， I-SP释放到培养液中的量增加了25倍，高于平均控制值。射频病变放置在雄性大鼠正中隆起-弓形区引起显著(p<。在AP和PP中I-SP的降低。这些降低与血浆催乳素值呈负相关。血浆催乳素升高作为病变完全性的指标。我们得出结论：1)大鼠垂体含有I-SP，通过其免疫和色谱行为来评估；2)K+去极化是体外释放AP I-SP的有效刺激因子；3)me -弓形区对维持大鼠垂体I-SP水平很重要。
Two distinct carboxy-terminus-directed anti-substance P (SP) sera (R-1C and R-6G) were used to characterize immunoreactive SP (I-SP) in acetic acid extracts of anterior pituitary (AP) and posterior pituitary (PP) glands of adult male rats. The tissue concentrations of I-SP measured by R-1C and R-6G were comparable. The contents of I-SP were 600–1150 pg/AP and 25–52 pg/PP. I-luteinizing hormone releasing hormone and I-somatostatin (I-SOM) were undetectable in AP extracts, but PP extracts contained the equivalents of 325–785 pg I-SOM/gland. Serial dilutions of AP and PP extracts produced displacement curves with both SP antisera that were parallel to the respective synthetic SP standard and hypothalamic extract displacement curves. Gel filtrations of AP and PP extracts on a Sephadex G-25 column produced I-SP peaks eluting in the same fractions as synthetic SP and hypothalamic I-SP. However, the AP I-SP profile also revealed a side peak migrating between the void volume and the major I-SP peak. Neither immunoreactive species in the AP extract were eliminated when eluted with 6.0 M guanidine HCl, a strong denaturing agent. In vitro incubation of paired anterior hemipituitaries for 30 min in the presence of a 56 mM K+ concentration resulted in a significant (p<. 0001), 25-fold increase in the release of I-SP into the incubation medium above the mean control value. Radiofrequency lesions placed in the median eminence-arcuate region of male rats caused a significant (p<. 001) reduction of I-SP in both the AP and PP. These reductions were inversely related to the plasma prolactin values. The elevation in plasma prolactin was taken as an index of completeness of lesions. We conclude that: 1) the rat pituitary contains I-SP as assessed by its immunologic and chromatographic behavior, 2) K+ depolarization is a potent stimulator of the release of AP I-SP in vitro, 3) the ME-arcuate region is important for the maintenance of pituitary I-SP levels in the rat.