Coupling of manganese peroxidase-mediated lipid peroxidation with destruction of nonphenolic lignin model compounds and 14C-labeled lignins.
Coupling of manganese peroxidase-mediated lipid peroxidation with destruction of nonphenolic lignin model compounds and 14C-labeled lignins.
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锰过氧化物酶介导的脂质过氧化与非酚木质素模型化合物和 14C 标记木质素的破坏的耦合。
DOI：
10.1006/bbrc.1999.0742
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发表时间：
1999
影响因子：
3.1
通讯作者：
A. Hatakka
中科院分区：
文献类型：
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作者：
A. Kapich;M. Hofrichter;T. Vares;A. Hatakka
亚油酸是木腐菌脂类中的主要不饱和脂肪酸(UFA)，由白腐菌Plebia Radiata产生的锰过氧化物酶(MNP)通过过氧化机制氧化。过氧化氢能显著促进细胞的过氧化。作为脂质过氧化底物的不饱和脂肪酸和乳化水不溶组分的表面活性剂对于MNP催化破坏非酚β-O-4连接的木质素模型化合物(LMC)是必不可少的。此外，这两个组分都促进了14C标记的合成木质素和14C标记的麦草在MNP催化下的矿化。在吐温80既是表面活性剂又是不饱和脂肪酸来源的反应体系中，获得了很高的破坏水平。在与MNP和Tween80反应体系中，亚油酸的存在进一步提高了LMC破坏和木质素矿化的速度和水平。结果表明，脂质过氧化在木腐菌降解木质素的过程中可能起重要作用，并支持木质素降解过程之间的耦合假说。
Linoleic acid, the predominant unsaturated fatty acid (UFA) in the lipids of wood-rotting fungi, was oxidized by manganese peroxidase (MnP) from the white-rot fungus Phlebia radiata through a peroxidation mechanism. The peroxidation was markedly stimulated by hydrogen peroxide. UFAs that are substrates for lipid peroxidation and surfactants that emulsify water-insoluble components were essential for the MnP-catalyzed destruction of a nonphenolic beta-O-4-linked lignin model compound (LMC). Moreover, both components stimulated the MnP-catalyzed mineralization of 14C-labeled synthetic lignin and 14C-labeled wheat straw. A high level of destruction was obtained in reaction systems with Tween 80 acting both as surfactant and source of UFAs. The presence of the linoleic acid in reaction systems with MnP and Tween 80 additionally enhanced rate and level of LMC destruction and lignin mineralization. The results indicate that lipid peroxidation may play an important role in lignin biodegradation by wood-rotting basidiomycetes and support the hypothesis of coupling between the processes.
Tsunewaki, K.：农业和林业生物技术。
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