Tgf-beta1, Tgf-beta2, Tgf-beta3 and Msx2 expression is elevated during frontonasal suture morphogenesis and during active postnatal facial growth.
Tgf-beta1, Tgf-beta2, Tgf-beta3 and Msx2 expression is elevated during frontonasal suture morphogenesis and during active postnatal facial growth.
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DOI：
10.1034/j.1600-0544.2002.02227.x
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发表时间：
2002-11-01
影响因子：
3.1
通讯作者：
Opperman, L A
中科院分区：
文献类型：
--
作者：
Adab, K;Sayne, J R;Opperman, L A
目的：据推测，调控面缝形态发生是类似的颅缝，具有类似的调控分子的表达，支配缝的形成和开放。本研究旨在观察不同发育阶段大鼠额鼻缝（FN）的形态学特征，并探讨转化生长因子β 1（TGF β 1）在FN中的表达及其时空变化（Tgf-β 1）、Tgf-β 2、Tgf-β 3和Msx 2 mRNA。美国德克萨斯州达拉斯贝勒牙科学院，德克萨斯A&M大学系统健康科学中心生物医学科学系。方法：取SD大鼠胎龄16 ~生后20 d的FN缝合组织，进行组织切片和RNA提取。基因表达测定的RT-PCR产物上运行的琼脂糖gels.Results密度分析：FN缝发展稍晚于颅缝，并显示随着时间的推移，颅缝相比，复杂性增加。FN缝线与鼻囊软骨密切相关，中间层为软骨膜和骨膜。FN缝合组织中TGF-β的表达模式与颅缝中观察到的相似。然而，与颅缝相比，Tgf-betas的mRNA和蛋白质随着时间的推移而出现差异表达。在FN缝线中，TGF-β mRNA水平在缝线形态发生期间和出生后早期缝线的活跃骨生长期间均升高。Msx 2 mRNA的表达在出生前和出生后的时期，类似TGF-β mRNA expression.CONCLUSION：TGF-β和Msx 2是存在于面部缝线类似颅缝，但随着时间的推移差异表达，可能反映了不同的骨生长率从这些缝。
OBJECTIVES: It is hypothesized that regulation of facial suture morphogenesis is similar to that of cranial sutures, with expression of similar regulatory molecules, governing suture formation and patency. The present study was designed to characterize the morphology of the frontonasal (FN) suture of the rat at different developmental stages and to investigate the presence and temporal-spatial expression of transforming growth factor-beta 1 (Tgf-beta1), Tgf-beta2, Tgf-beta3 and Msx2 mRNA within these structures.SETTING AND SAMPLE POPULATION: The Department of Biomedical Sciences at Texas A&M University System Health Science Center, Baylor College of Dentistry, Dallas, TX USA. Histological sections and RNA isolated from FN suture tissues of Sprague-Dawley rats, aged embryonic day 16 through postnatal day 20.METHOD: Sections were examined after immunohistochemical staining. Gene expression was determined by densitometric analysis of RT-PCR products run on agarose gels.RESULTS: FN sutures develop slightly later than cranial sutures and show increased complexity over time when compared to cranial sutures. FN sutures were closely associated with the nasal capsular cartilage, with intervening layers of perichondrium and periosteum. The pattern of expression of Tgf-betas within the FN suture tissues was similar to that seen in the cranial sutures. However, mRNA and protein of the Tgf-betas were differentially expressed over time compared to cranial sutures. In FN sutures, Tgf-beta mRNA levels were elevated both during the period of suture morphogenesis and during active bone growth from the suture in the early postnatal period. Msx2 mRNA expression was elevated in both the prenatal and postnatal periods, similar to Tgf-beta mRNA expression.CONCLUSION: Tgf-beta and Msx2 are present in facial sutures similar to cranial sutures, but are differentially expressed over time, perhaps reflecting different bone growth rates from these sutures.