HBI1 acts downstream of ERECTA and SWR1 in regulating inflorescence architecture through the activation of the brassinosteroid and auxin signaling pathways
HBI1 acts downstream of ERECTA and SWR1 in regulating inflorescence architecture through the activation of the brassinosteroid and auxin signaling pathways
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HBI1 作用于 ERECTA 和 SWR1 下游，通过激活油菜素类固醇和生长素信号通路来调节花序结构
DOI：
10.1111/nph.16840
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发表时间：
2020-09-01
期刊：
影响因子：
9.4
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
--
作者：
Cai, Hanyang;Chai, Mengnan;Qin, Yuan
花序结构直接影响植物的繁殖成功率和产量，反映了花序分生组织的活力和花梗的长度。在拟南芥中，ERECTA （ER）信号通路和SWR1染色质重塑复合体通过促进paclobutrazol RESISTANCE（PRE）基因家族的表达，共同调控花序结构。然而，pres如何调控花序结构尚不清楚。rna测序和染色质免疫沉淀结合定量PCR分析。进一步研究了BEE2与IBH1相互作用的同源基因（HBI1）与SWR1-ER-MPK6通路在控制花序结构中的遗传相互作用。目前的研究结果支持athbi1在SWR1和ER通路中发挥pres下游的作用，通过促进花梗伸长来调节花序结构。具体来说，它结合油菜素内酯（brassinosteroids， BR）生物合成基因ecyp85a2的启动子和一系列生长素相关基因，包括生长素反应因子arf3，并促进它们的表达。反过来，ARF3还可以结合生长素信号基因和cyp85a2，激活它们的表达，促进花梗伸长。我们的研究提供了证据，证明SWR1和ER对花序结构的调控涉及HBI1调控枢纽及其激活BR和生长素激素途径。
Inflorescence architecture critically influences plant reproductive success and crop yield, and it reflects the activity of the inflorescence meristem and pedicel length. InArabidopsis thaliana, the ERECTA (ER) signaling pathway and the SWR1 chromatin remodeling complex jointly regulate inflorescence architecture by promoting the expression of thePACLOBUTRAZOL RESISTANCE(PRE) gene family. However, howPREs regulate inflorescence architecture remains unclear. RNA-sequencing and chromatin immunoprecipitation coupled with quantitative PCR analyses were performed. Genetic interactions betweenHOMOLOG OF BEE2 INTERACTING WITH IBH1(HBI1) and the SWR1-ER-MPK6 pathway in the control of inflorescence architecture were further studied. The present findings support thatHBI1functions downstream ofPREs in the SWR1 and ER pathways to regulate inflorescence architecture by promoting pedicel elongation. Specifically, it binds to the promoters of the brassinosteroid (BR) biosynthesis geneCYP85A2and a series of auxin-related genes, including auxin response factorARF3, and promotes their expression. In turn, ARF3 can also bind to auxin signaling genes as well asCYP85A2to activate their expression and promote pedicel elongation. Our study provides evidence that inflorescence architecture regulation by SWR1 and ER involves the HBI1 regulatory hub and its activation of both the BR and auxin hormone pathways.