Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae
Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae
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DOI：
10.1111/j.1574-6968.2010.01931.x
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发表时间：
2010-05-01
影响因子：
2.1
通讯作者：
Yamasaki, Shinji
中科院分区：
文献类型：
--
作者：
Chatterjee, Shruti;Asakura, Masahiro;Yamasaki, Shinji
使用天然化合物作为毒力因子产生的抑制剂是克服病原菌耐药性增加的新方法。在这项研究中，我们研究了红辣椒（辣椒）是否含有任何这样的化合物，可以抑制霍乱弧菌的霍乱毒素（CT）的生产。我们发现红辣椒的甲醇提取物可以抑制最近出现的霍乱弧菌O 1 El Tor变异株的CT产生，而不影响其活力。有趣的是，辣椒素，红辣椒的一种被充分研究的活性成分，也显著抑制了属于各种血清群（包括变体）的霍乱弧菌菌株中的CT产生。实时荧光定量RT-PCR检测结果表明，辣椒素能有效抑制ctxA、tcpA和toxT基因的转录，但对toxR和toxS基因的转录无影响。相反，辣椒素显着增强的hns基因的转录，其产品是已知的负调节ctxAB，tcpA和toxT基因的转录。这些结果表明，辣椒素可能作为一个有效的阻遏CT的生产可能通过增强转录的hns。
The use of natural compounds as inhibitory agents for virulence factor production is a new approach to overcome increased antimicrobial resistance in pathogenic bacteria. In this study, we examined whether red chilli (Capsicum annuum) contains any such compound(s) that can repress the cholera toxin (CT) production in Vibrio cholerae. We found that the methanol extract of red chilli could inhibit CT production in recently emerged V. cholerae O1 El Tor variant strains without affecting their viability. Interestingly, capsaicin, a well-studied active component of red chilli, also drastically inhibited CT production in V. cholerae strains belonging to various serogroups including variants. Real-time quantitative reverse transcription-PCR assay revealed that capsaicin effectively repressed the transcription of ctxA, tcpA and toxT genes, but not of toxR and toxS genes. On the contrary, capsaicin significantly enhanced the transcription of the hns gene, the product of which is known to regulate negatively the transcription of ctxAB, tcpA and toxT genes. These results suggest that capsaicin might act as a potent repressor for CT production possibly by enhancing the transcription of hns.