Physical characteristics of small intestinal submucosa scaffolds are location-dependent
Physical characteristics of small intestinal submucosa scaffolds are location-dependent
复制标题
DOI：
10.1002/jbm.a.30268
复制
发表时间：
2005-04-01
影响因子：
4.9
通讯作者：
Madihally, SV
中科院分区：
文献类型：
--
作者：
Raghavan, D;Kropp, BP;Madihally, SV
使用可生物降解支架作为工程新组织的替代方案已成为各种移植方案中有吸引力的候选方案。特别是小肠粘膜下层（SIS），一种来自小肠的致密结缔组织基质，由于其许多有利的特性而受到关注。然而，在影响再生过程的大规模制剂中获得可靠的、可重复的产品，限制了SIS的使用。为了更好地理解SIS的异质性，本研究侧重于评估从远端和近端收获并在室内加工（称为手工制作）的基质的物理特性的位置依赖性变化。此外，将结果与市售的机器制造的Cook (R) SIS进行比较。在潮湿条件下，比较了单调加载和循环加载的拉伸性能。此外，使用定制的腔室分析了这些膜对尿素的渗透性，并通过扫描电子显微镜分析了微结构。这些结果表明，相对于其他样品，远端样品更具弹性，对尿素的渗透性更低。然而，每个样品的渗透性都是方向依赖性的，即在所有样品中，粘膜到浆膜方向的渗透性都比鼻粘膜到粘膜方向的渗透性要低。Cook (R) SIS更易受周期性载荷影响，承载能力范围较短。综上所述，结果表明，SIS的物理特征与位置有关。(c) 2005 Wiley期刊有限公司
Using biodegradable scaffolds as an alternative to engineer new tissues has become an attractive candidate in various transplantation protocols. In particular, small intestinal submucosa (SIS), a dense connective matrix harvested from the small intestine, has gained attention due to a number of favorable properties. However, use of SIS is constrained by obtaining reliable, reproducible products in large-scale preparations that affect the regenerative process. To better understand the heterogeneous nature of SIS, this study focused on evaluating the location-dependent alterations in the physical characteristics of the matrices harvested from distal and proximal ends and processed inhouse (referred as hand-made). Additionally, results were compared with a commercially available machine-made Cook (R) SIS. Tensile properties during monotonic loading and cyclical loading were compared in wet conditions. Further-more, permeability of these membranes to urea was analyzed using a custom-built chamber, and the microarchitecture was analyzed via scanning electron microscopy. These results showed that distal samples were more elastic and less permeable to urea relative to other samples. However, permeability in each sample was direction-dependent, that is, mucosal to serosal direction was less permeable compared to sorasal to mucosal direction in all the samples. Cook (R) SIS was more susceptible to cyclical loading and had a shorter range of load carrying capacity. In summary, results show that physical characteristics of SIS are location-dependent. (c) 2005 Wiley Periodicals, Inc.