Decellularized allogeneic intervertebral disc: natural biomaterials for regenerating disc degeneration.
Decellularized allogeneic intervertebral disc: natural biomaterials for regenerating disc degeneration.
复制标题
脱细胞同种异体椎间盘：用于再生椎间盘退变的天然生物材料。
DOI：
10.18632/oncotarget.7735
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发表时间：
2016-03-15
期刊：
影响因子：
--
通讯作者：
Fan S
中科院分区：
文献类型：
--
作者：
Lin X;Fang X;Wang Q;Hu Z;Chen K;Shan Z;Chen S;Wang J;Mo J;Ma J;Xu W;Qin A;Fan S
椎间盘退变与背痛和椎间盘突出有关。本研究建立了一种改良的椎间盘脱细胞方法，并制备了椎间盘细胞外基质。将骨髓间充质干细胞（mesenchymal stem cells，MSCs）和人退变椎间盘细胞（human degenerative IVD cells，IVD）分别培养3、7、14和21 d，植入兔皮下，并将ECM微粒注入退变椎间盘，评价脱细胞IVD的生物学安全性和有效性。在这里，我们证明了细胞成分可以在脱细胞后完全去除，并最大限度地保留天然IVD的结构和生物力学。我们发现，同种异体ECM在体内没有引起任何明显的炎症反应，在体外也没有发现细胞毒性。体外实验表明，体外培养的细胞外基质可诱导骨髓间充质干细胞分化为体外培养的细胞样细胞。此外，同种异体ECM微粒在体内治疗兔椎间盘退变是有效的。总之，我们的研究开发了一种优化的IVD脱细胞方法，我们证明脱细胞IVD是安全有效的治疗退行性椎间盘疾病。
Intervertebral disc degeneration is associated with back pain and disc herniation. This study established a modified protocol for intervertebral disc (IVD) decellularization and prepared its extracellular matrix (ECM). By culturing mesenchymal stem cells (MSCs)(3, 7, 14 and 21 days) and human degenerative IVD cells (7 days) in the ECM, implanting it subcutaneously in rabbit and injecting ECM microparticles into degenerative disc, the biological safety and efficacy of decellularized IVD was evaluated both in vitro and in vivo. Here, we demonstrated that cellular components can be removed completely after decellularization and maximally retain the structure and biomechanics of native IVD. We revealed that allogeneic ECM did not evoke any apparent inflammatory reaction in vivo and no cytotoxicity was found in vitro. Moreover, IVD ECM can induce differentiation of MSCs into IVD-like cells in vitro. Furthermore, allogeneic ECM microparticles are effective on the treatment of rabbit disc degeneration in vivo. In conclusion, our study developed an optimized method for IVD decellularization and we proved decellularized IVD is safe and effective for the treatment of degenerated disc diseases.