Bcl-2 and porin follow different pathways of TOM-dependent insertion into the mitochondrial outer membrane
Bcl-2 and porin follow different pathways of TOM-dependent insertion into the mitochondrial outer membrane
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DOI：
10.1016/s0022-2836(02)00995-6
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发表时间：
2002-11-01
影响因子：
5.6
通讯作者：
Rassow, J
中科院分区：
文献类型：
--
作者：
Motz, C;Martin, H;Rassow, J
Bcl-2基因编码一个26 kDa的蛋白，其功能是细胞凋亡的中央调节。在这里，我们以酿酒酵母为模式生物，研究了Bcl2α进入线粒体外膜的途径。我们发现，Bcl-2α与线粒体输入受体Tom20的相互作用依赖于羧基末端疏水膜锚附近的两个带正电的赖氨酸残基。这些残基的靶向功能不依赖于Tom22。随后将Bcl-2α插入线粒体外膜不需要Tom5或Tom40，表明Bcl-2α绕过了一般输入孔(GIP)。BCL-2α与线粒体TOM复合体的成分显示出一种独特的相互作用模式，表明至少有两条不同的途径从输入受体Tom20进入线粒体外膜。(C)2002爱思唯尔科学有限公司。保留所有权利。
The bcl-2 gene encodes a 26 kDa protein which functions as a central regulator of apoptosis. Here we investigated the pathway of Bcl-2alpha into the mitochondrial outer membrane using the yeast Saccharomyces cerevisiae as a model organism. We found that interactions of Bcl-2alpha with the mitochondrial import receptor Tom20 are dependent on two positively charged lysine residues in the immediate vicinity of the carboxy-terminal hydrophobic membrane anchor. The targeting function of these residues is independent of Tom22. Subsequent insertion of Bcl-2alpha into the mitochondrial outer membrane does not require Tom5 or Tom40, indicating that Bcl-2alpha bypasses the general import pore (GIP). Bcl-2alpha shows a unique pattern of interactions with the components of the mitochondrial TOM complex, demonstrating that at least two different pathways lead from the import receptor Tom20 into the mitochondrial outer membrane. (C) 2002 Elsevier Science Ltd. All rights reserved.