B-CELL STIMULATORY FACTOR-I INTERLEUKIN-4 MESSENGER-RNA IS EXPRESSED BY NORMAL AND TRANSFORMED MAST-CELLS
B-CELL STIMULATORY FACTOR-I INTERLEUKIN-4 MESSENGER-RNA IS EXPRESSED BY NORMAL AND TRANSFORMED MAST-CELLS
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DOI：
10.1016/0092-8674(87)90339-4
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发表时间：
1987-08-28
期刊：
影响因子：
64.5
通讯作者：
PAUL, WE
中科院分区：
文献类型：
--
作者：
BROWN, MA;PIERCE, JH;PAUL, WE
BSF - 1/白细胞介素 - 4是活化T细胞的产物，具有多种影响大多数造血谱系细胞的生物学活性。其中包括BSF - 1协同刺激肥大细胞生长以及调节IgE产生的能力。在此我们证明，大多数转化的肥大细胞系以及5种白细胞介素 - 3依赖性非转化肥大细胞系都表达BSF - 1 mRNA。在转化的肥大细胞的上清液中检测到了BSF - 1活性，包括增强白细胞介素 - 3依赖性肥大细胞生长的能力。BSF - 1作为一种肥大细胞生长因子的作用、转化的肥大细胞对其组成性产生以及这些细胞中大多数不产生白细胞介素 - 3，这些都增加了BSF - 1可能作为某些转化的肥大细胞的自分泌生长因子的可能性。此外，非转化细胞产生BSF - 1 mRNA表明肥大细胞可能是这种因子的一个重要生理来源。
BSF-1/interleukin-4, a product of activated T cells, has multiple biological activities that affect cells of most hematopoietic lineages. Among these is the ability of BSF-1 to costimulate the growth of mast cells and regulate the production of IgE. We demonstrate here that BSF-1 mRNA is expressed by a majority of transformed mast cell lines and by 5 IL-3 dependent non-transformed mast cell lines. BSF-1 activity, including the ability to enhance the growth of IL-3 dependent mast cells, was detected in the supernatants of transformed mast cells. The role of BSF-1 as a mast cell growth factor, its constitutive production by transformed mast cells, and the lack of IL-3 production by most of these cells raise the possibility that BSF-1 may act as an autocrine growth factor for some transformed mast cells. Furthermore, production of BSF-1 mRNA by nontransformed cells indicates that mast cells may be an important physiologic source of this factor.