Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.
Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.
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增加细胞内 Na 浓度可诱导前 B 淋巴细胞细胞系分化。
DOI：
10.1073/pnas.80.24.7547
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发表时间：
1983
影响因子：
11.1
通讯作者：
Cantley,LC
中科院分区：
文献类型：
--
作者：
Rosoff,PM;Cantley,LC
脂多糖（LPS）（一种多克隆B细胞有丝分裂原）诱导前B细胞肿瘤系70 Z/3分化的能力可以通过增加细胞内Na+浓度的药物来模拟。药理学上增加细胞Na+含量与莫能菌素，钠离子载体，或哇巴因，Na+，K+-ATP酶的特异性抑制剂，诱导表面IgM在这些细胞中的表达。我们已经表明，LPS刺激Na+的摄取通过阿米洛利敏感的途径。这些结果表明，LPS诱导表面IgM表达的基本作用是激活Na+摄取系统。
The ability of lipopolysaccharide (LPS), a polyclonal B-cell mitogen, to induce differentiation in the pre-B cell tumor line 70Z/3 can be mimicked by drugs that increase the intracellular Na+ concentration. Pharmacologically increasing the cellular Na+ content with monensin, a sodium ionophore, or ouabain, a specific inhibitor of Na+, K+-ATPase, induces surface IgM expression in these cells. We have shown that LPS stimulates uptake of Na+ through an amiloride-sensitive pathway. These results show that the essential action of LPS to induce surface IgM expression is activation of a Na+ uptake system.