Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast.
Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast.
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YHM1（编码线粒体载体蛋白）在酵母铁分布中的作用。
DOI：
10.1042/bj20031387
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Dancis,Andrew
中科院分区：
文献类型：
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作者：
Lesuisse,Emmanuel;Lyver,EliseR;Knight,SimonAB;Dancis,Andrew
线粒体载体蛋白是酿酒酵母中的一个大蛋白家族，由35个成员组成。这个蛋白质家族的成员被证明可以运输不同的底物，从细胞质到线粒体或线粒体到细胞质，尽管许多家族成员没有指定的底物。我们推测，这些转运蛋白中是否有一个或多个会在铁代谢中发挥作用。对每个缺失单一线粒体载体蛋白的单倍体酵母菌株进行了铁稳态变化分析。缺失YHM1的菌株的特征是表面铁还原酶和高亲和力铁转运活性增加和调节不当。来自不同来源的铁载体的摄取也增加了，这些影响依赖于AFT1铁传感器调节器。YHM1突变株转化为Rho°，与线粒体铁积累引起的线粒体DNA二次损伤一致。事实上，在Δyhm1突变体中，发现铁积累在线粒体中。在分离的线粒体中，使用内源可用金属和添加的卟啉测量，累积的铁显示用于血红素合成的有效性降低。Δyhm1突变体的表型表明，这种线粒体转运蛋白在细胞铁稳态中发挥了作用。
Mitochondrial carrier proteins are a large protein family, consisting of 35 members inSaccharomyces cerevisiae. Members of this protein family have been shown to transport varied substrates from cytoplasm to mitochondria or mitochondria to cytoplasm, although many family members do not have assigned substrates. We speculated whether one or more of these transporters will play a role in iron metabolism. Haploid yeast strains each deleted for a single mitochondrial carrier protein were analysed for alterations in iron homoeostasis. The strain deleted forYHM1was characterized by increased and misregulated surface ferric reductase and high-affinity ferrous transport activities. Siderophore uptake from different sources was also increased, and these effects were dependent on theAFT1iron sensor regulator. Mutants ofYHM1converted into rho°, consistent with secondary mitochondrial DNA damage from mitochondrial iron accumulation. In fact, in the Δyhm1mutant, iron was found to accumulate in mitochondria. The accumulated iron showed decreased availability for haem synthesis, measured in isolated mitochondria using endogenously available metals and added porphyrins. The phenotypes of Δyhm1mutants indicate a role for this mitochondrial transporter in cellular iron homoeostasis.