HISTONE-H3 TRANSCRIPTION IN SACCHAROMYCES-CEREVISIAE IS CONTROLLED BY MULTIPLE CELL-CYCLE ACTIVATION SITES AND A CONSTITUTIVE NEGATIVE REGULATORY ELEMENT
HISTONE-H3 TRANSCRIPTION IN SACCHAROMYCES-CEREVISIAE IS CONTROLLED BY MULTIPLE CELL-CYCLE ACTIVATION SITES AND A CONSTITUTIVE NEGATIVE REGULATORY ELEMENT
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DOI：
10.1128/mcb.12.12.5455
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发表时间：
1992-12-01
影响因子：
5.3
通讯作者：
SMITH, MM
中科院分区：
文献类型：
--
作者：
FREEMAN, KB;KARNS, LR;SMITH, MM
研究了酿酒酵母组蛋白H3和H4基因的启动子的顺式作用DNA序列元件调节转录和细胞分裂周期控制。缺失和接头破坏突变确定了两类调节元件：多个细胞周期激活（CCA）位点和负调节位点（NRS）。重复的19-bp CCA位点存在于拷贝I和拷贝II组蛋白H3-H4启动子中，排列为由45和68 bp分开的反向重复序列。CCA位点是在细胞分裂周期控制下激活转录所必需的并且是足够的。单个CCA位点提供细胞周期控制，但是弱转录激活因子，而包含两个CCA位点的反向重复序列提供强转录激活和细胞分裂周期控制。在组蛋白H3-H4启动子I拷贝中鉴定出NRS。NRS的缺失或破坏增加了组蛋白H3启动子活性的水平，但没有改变细胞分裂周期的转录周期。当CCA位点从组蛋白启动子中删除时，NRS元件不能在剩余的基础转录水平上赋予细胞分裂周期控制。当NRS元件被插入到一个外来的报告基因的启动子，转录组成性抑制，并没有获得细胞周期调控。
The promoters of the Saccharomyces cerevisiae histone H3 and H4 genes were examined for cis-acting DNA sequence elements regulating transcription and cell division cycle control. Deletion and linker disruption mutations identified two classes of regulatory elements: multiple cell cycle activation (CCA) sites and a negative regulatory site (NRS). Duplicate 19-bp CCA sites are present in both the copy I and copy II histone H3-H4 promoters arranged as inverted repeats separated by 45 and 68 bp. The CCA sites are both necessary and sufficient to activate transcription under cell division cycle control. A single CCA site provides cell cycle control but is a weak transcriptional activator, while an inverted repeat comprising two CCA sites provides both strong transcriptional activation and cell division cycle control. The NRS was identified in the copy I histone H3-H4 promoter. Deletion or disruption of the NRS increased the level of the histone H3 promoter activity but did not alter the cell division cycle periodicity of transcription. When the CCA sites were deleted from the histone promoter, the NRS element was unable to confer cell division cycle control on the remaining basal level of transcription. When the NRS element was inserted into the promoter of a foreign reporter gene, transcription was constitutively repressed and did not acquire cell cycle regulation.