Evaluation of malignant and benign gastric biopsy specimens by mRNA expression profile and multivariate statistical methods
Evaluation of malignant and benign gastric biopsy specimens by mRNA expression profile and multivariate statistical methods
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DOI：
10.1002/cyto.b.20189
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发表时间：
2007-09-01
影响因子：
3.4
通讯作者：
Tulassay, Zsolt
中科院分区：
文献类型：
--
作者：
Galamb, Orsolya;Sipos, Ferenc;Tulassay, Zsolt
背景：胃活检的 mRNA 表达阵列和多变量统计分析可以深入了解局部改变的分子生物学基础，支持基于表达的形态学改变的识别。方法：从 11 例糜烂性胃炎（EG）、5 例腺癌（GC）、11 例萎缩性胃炎（AG）患者中收集胃活检，分离总 RNA，T7 使用商业玻璃阵列（Clontech，美国）对 1047 个 mRNA 进行扩增和表达分析。微阵列质量控制后，可从 7 EG、4 GC 和 5 AG 获得适用数据。进行多变量统计和细胞功能分析。采用实时RT-PCR和免疫组织化学进行验证。结果：GC的特征是过度调节的v-raf、v-erb-a、BCL2相关的athanogene、immediate-early-response-3、Polo样激酶、CDK-2、cyclin-C、Pin1基因，以及下调的ADP-核糖基转移酶、唾液酸蛋白和DCC。 AG病例的PDGF-受体、TGF-β-受体-3增加，死亡相关蛋白-3、β-1-连环蛋白、拓扑异构酶-1水平降低。在 EG 中，发现 IGF-receptor-1、CD9、tiransferrin 受体、整合素上调，而 keratin-5、caspase-4 表达不足。判别分析可以使用四个参数正确地重新分类所有样本。结论：胃活检的 mRNA 表达阵列分析在评估局部胃改变时产生了先前已知的和新的数据。 (c) 2007 年临床细胞计数学会。
Background: mRNA expression array and multivariate statistical analysis of gastric biopsies can yield insight into the molecular biology basis of local alterations, supporting expression-based identification of morphological alterations.Methods: From 11 patients with erosive gastritis(EG), 5 with adenocarcinoma (GC), 11 with atrophic gastritis (AG) gastric biopsies were collected, total RNA isolated, T7 amplification and expression analysis of 1047 mRNAs was performed using commercial glass arrays (Clontech, USA). After microarray quality control, applicable data were available from 7 EG, 4 GC, and 5 AG. Multivariate statistical and cell functional analysis were performed. Real-time RT-PCR and immunohistochemistry were used for validation.Results: GC was characterized by overregulated v-raf, v-erb-a, BCL2-associated- athanogene, immediate-early-response-3, Polo-like kinase, CDK-2, cyclin-C, Pin1 genes, and downregulated ADP-ribosyltransferase, sialophorin and DCC. AG cases had increased PDGF-receptor, TGF-beta-receptor-3, and decreased death-associated-protein-3, beta-1-catenin, topoisomerase-1 levels. In EG upregulation of IGF-receptor-1, CD9, tiransferrin receptor, integrins, and underexpression of keratin-5, caspase-4 was found. Discriminant analysis could reclassify all samples correctly using four parameters.Conclusions: mRNA expression array analysis of gastric biopsies yields previously known and new data in the evaluation of local gastric alterations. (c) 2007 Clinical Cytometry Society.