THE APPLICATION OF PERPENDICULAR AND FORWARD LIGHT SCATTER TO ASSESS NUCLEAR AND CELLULAR MORPHOLOGY
THE APPLICATION OF PERPENDICULAR AND FORWARD LIGHT SCATTER TO ASSESS NUCLEAR AND CELLULAR MORPHOLOGY
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DOI：
10.1002/cyto.990050513
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
COFFEY, DS
中科院分区：
文献类型：
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作者：
BENSON, MC;MCDOUGAL, DC;COFFEY, DS
在多参数方法中采用了周向和前向光散射来区分可移植的Dunning R3327大鼠前列腺腺癌的各种线。周围光散射与核的大小和形状相关。前向光散射和垂直光散射信号的平均强度被证明随着细胞分化程度降低而增加。垂直光散射和前向光散射的组合允许区分组织学上无法区分的肿瘤，因此可能有助于建立癌症分级系统。
Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter correlated with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well-differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.