LITTLELEAF (LL) encodes a WD40 repeat domain-containing protein associated with organ size variation in cucumber.
LITTLELEAF (LL) encodes a WD40 repeat domain-containing protein associated with organ size variation in cucumber.
复制标题
LITTLELEAF (LL) 编码与黄瓜器官大小变化相关的包含 WD40 重复结构域的蛋白质。
DOI：
10.1111/tpj.13991
复制
发表时间：
2018
期刊：
影响因子：
7.2
通讯作者：
Weng Yiqun
中科院分区：
文献类型：
--
作者：
Yang Luming;Liu Hanqiang;Zhao Jianyu;Pan Yupeng;Cheng Siyuan;Lietzow Calvin D;Wen Changlong;Zhang Xiaolan;Weng Yiqun
植物采用严格的遗传控制来整合内在的生长信号和环境线索，使器官生长到预定的大小。已鉴定出许多有助于细胞增殖和/或细胞扩张以及随后的器官大小控制的基因，但其调节途径却知之甚少。在这里，我们描述了一个 Cucumberlittleleaf(ll) 突变体，它比野生型表现出更小的器官尺寸，但有更多的侧枝。器官尺寸变小是由于细胞数量和细胞尺寸的减少。数量性状基因座（QTL）分析揭示了果实大小、果实和种子重量以及侧枝数量的主效QTL的共定位，LL基因座表明了突变的多效性。我们证明LL是拟南芥STERILE APETALA（SAP）的直系同源物，编码含有WD40重复结构域的蛋白质；该突变蛋白与野生型蛋白的不同之处在于第二个 WD40 重复序列中的单个氨基酸取代 (W264G)。 W264 在所检查的 34 个维管植物基因组中是保守的。系统发育分析表明，L起源于开花植物出现之前，但在禾本科植物基因组谱系中丢失了。 LL在器官大小控制中的功能通过其在转基因黄瓜中的过表达和在拟南芥中的异位表达而得到证实。 Landllbulks 中的转录组分析揭示了 LL 介导的器官大小变化的复杂调节网络，其中涉及几种已知的器官大小调节因子和相关途径。数据支持 LL 作为黄瓜器官大小控制和侧枝发育的重要参与者。
Plants employ tight genetic control to integrate intrinsic growth signals and environmental cues to enable organs to grow to a defined size. Many genes contributing to cell proliferation and/or cell expansion, and consequently organ size control, have been identified, but the regulatory pathways are poorly understood. Here we have characterized a cucumberlittleleaf(ll) mutant which exhibits smaller organ sizes but more lateral branches than the wild type. The small organ size inllwas due to a reduction of both cell number and cell size. Quantitative trait locus (QTL) analyses revealed co‐localization of major‐effect QTLs for fruit size, fruit and seed weight, as well as number of lateral branches, with theLLlocus indicating pleiotropic effects of thellmutation. We demonstrate thatLLis an ortholog of ArabidopsisSTERILE APETALA(SAP) encoding a WD40 repeat domain‐containing protein; the mutant protein differed from the wild type by a single amino acid substitution (W264G) in the second WD40 repeat. W264 was conserved in 34 vascular plant genomes examined. Phylogenetic analysis suggested thatLLoriginated before the emergence of flowering plants but was lost in the grass genome lineage. The function ofLLin organ size control was confirmed by its overexpression in transgenic cucumbers and ectopic expression in Arabidopsis. Transcriptome profiling inLLandllbulks revealed a complex regulatory network forLL‐mediated organ size variation that involves several known organ size regulators and associated pathways. The data support LL as an important player in organ size control and lateral branch development in cucumber.