Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats.
Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats.
复制标题
DOI：
10.3390/metabo3030701
复制
发表时间：
2013-08-13
期刊：
影响因子：
4.1
通讯作者：
Maier CS
中科院分区：
文献类型：
--
作者：
Wickramasekara SI;Zandkarimi F;Morré J;Kirkwood J;Legette L;Jiang Y;Gombart AF;Stevens JF;Maier CS
本研究报告使用行波离子迁移率四极杆飞行时间（ToF）质谱的血浆代谢组学。在给予不同口服剂量的黄腐酚（一种啤酒花衍生的膳食补充剂）后，获得肥胖Zucker fa/fa大鼠的血浆代谢产物谱。使用Synapt G2高清晰度质谱（HDMS）仪器以正模式和负模式进行液相色谱耦合数据独立串联质谱（LC-MSE）和LC-离子迁移谱（IMS）-MSE采集。该方法使用并行交替的低能量和高能量碰撞光谱采集模式提供大鼠血浆中代谢物类别的鉴定。使用模式识别方法分析数据集。选择统计学显著性（p < 0.05且倍数变化（FC）阈值> 1.5）特征以鉴定上调/下调的代谢物类别。使用漂移范围软件可视化的离子迁移率数据提供了代谢物类别差异的图形读数。
This study reports on the use of traveling wave ion mobility quadrupole time-of-flight (ToF) mass spectrometry for plasma metabolomics. Plasma metabolite profiles of obese Zucker fa/fa rats were obtained after the administration of different oral doses of Xanthohumol; a hop-derived dietary supplement. Liquid chromatography coupled data independent tandem mass spectrometry (LC-MSE) and LC-ion mobility spectrometry (IMS)-MSE acquisitions were conducted in both positive and negative modes using a Synapt G2 High Definition Mass Spectrometry (HDMS) instrument. This method provides identification of metabolite classes in rat plasma using parallel alternating low energy and high energy collision spectral acquisition modes. Data sets were analyzed using pattern recognition methods. Statistically significant (p < 0.05 and fold change (FC) threshold > 1.5) features were selected to identify the up-/down-regulated metabolite classes. Ion mobility data visualized using drift scope software provided a graphical read-out of differences in metabolite classes.