A first-generation physical map of the human genome
A first-generation physical map of the human genome
复制标题
第一代人类基因组物理图谱
作者：
Daniel Cohen;I. Chumakov;J. Weissenbach
为了鉴定人类疾病基因，迫切需要一组跨越人类每条染色体的有序重叠克隆基因组DNA片段。这样的物理图谱也为研究基因组的结构和功能提供了独特的材料。因此，我们详尽地分析了CEPH酵母人工染色体（YAC）文库，其中包含33，000个克隆，其插入片段大小是单独确定的。这些YAC的平均长度为0.9兆碱基，覆盖了相当于10个单倍体基因组。几个映射技术相结合，以提供多个来源的结构信息，这些克隆。最后，用超过2，000个遗传标记筛选文库，所述遗传标记准均匀地分布在基因组的90%以上。这些结果应该允许科学界构建所有人类染色体的详细地图。此外，我们提出了一个数据分析策略，产生第一代的综合地图，涵盖了大部分的人类基因组。
SETS of ordered overlapping cloned genomic DNA fragments that span each of the human chromosomes are urgently needed for identification of human disease genes. Such a physical map also provides unique material to study the structure and function of the genome. We have therefore exhaustively analysed the CEPH yeast artificial chromosome (YAC) library, which contains 33,000 clones, whose insert size was individually determined. These YACs have an average length of 0.9 megabases and cover the equivalent of 10 haploid genomes. Several mapping techniques were combined to provide multiple sources of structural information for most of these clones. Finally, the library was screened with more than 2,000 genetic markers quasiuniformly distributed over 90% of the genome. These results should allow the scientific community to construct detailed maps of all human chromosomes. Moreover, we propose a data analysis strategy that produces a first-generation integrated map covering most of the human genome.
DOI：
10.1073/pnas.89.4.1388
发表时间：
1992-02-15
影响因子：
11.1
作者：
RIED, T;BALDINI, A;WARD, DC
通讯作者：
WARD, DC